控制性促排卵中AMH和InhibinB水平与IVF-ET结局的相关性分析

The correlation analysis of AMH and Inhibin B levels in controlled ovarian stimulation with pregnancy outcome in vitro fertilization-embryo transfer

目的探讨控制性促排卵过程中抗苗勒氏管激素(anti-müllerian hormone,AMH)和抑制素B(inhibin B,INHB)水平与体外受精(in vitro fertilization,IVF)/卵母细胞浆内单精子注射(intracytoplasmic sperm injection,ICSI)—胚胎移植(embryo transfer,ET)妊娠结局的相关性。方法选择2012年1月至2013年12月因输卵管因素和/或男方因素在我院生殖中心首次接IVF/ICSI-ET助孕患者为研究对象,共92个促排卵周期,检测促排卵周期中血清及取卵日卵泡液AMH和INHB水平,根据妊娠结局分为妊娠组和非妊娠组,比较两组患者的一般特点、内分泌激素水平、AMH和INHB水平、获卵数、受精率等促排卵相关参数,并进行相关性分析。结果在妊娠组和非妊娠组中,患者的一般情况、激素水平、血清及卵泡液AMH和INHB水平、获卵数、受精率等促排卵相关参数均无统计学差异(P>0.05);血清AMH和INHB水平及卵泡液AMH水平均与窦卵泡数、h CG日雌二醇水平、获卵数存在显著正相关(P<0.001)。结论 AMH和INHB水平可以反映促排卵周期中卵巢反应性,与IVF/ICSI-ET妊娠结局无直接相关性。

Objective：To study the correlation of anti-müllerian hormone（AMH）,inhibin B（INHB）in controlled ovarian stimulation and pregnancy outcome in patients undergoing in vitro fertilization（IVF）or intracytoplasmic sperm injection（ICSI）-embryo transfer（ET）therapy. Method：Women for first IVF/ICSI-ET therapy due to the tubal and/or male factors from January 2012 to December 2013 were included in this study. 92 controlled ovarian stimulation（COS）cycles were analyzed. AMH,INHB in serum and follicle fluid on oocytes retrieval day were detected. Women general characteristics,basal and h CG day hormones,AMH and INHB levels,retrieved oocytes,fertilization rate,transfer embryos,duration of COS,dosage of ganadotropins and pregnant outcomes were evaluated. Results：There was no significant difference on the general characteristics,AMH and INHB levels,retrieved oocytes and fertilization rate between pregnancy group and non-pregnancy group（P〉0.05）. There was a positive correlation of serum AMH and INHB,AMH in follicle fluid with retrieved oocytes and estradiol on h CG day（P〈0.001）. Conclusion：AMH and INHB level can reflect the ovarian response in ART cycles,but there was no direct correlation with pregnancy outcome.