家蚕血液肽聚糖识别蛋白的鉴定和生物信息学分析

Identification and Bioinformatics Analysis on Peptidoglycan Recognition Protein of Silkworm Haemolymph

采用双向电泳和质谱技术分析、鉴定了家蚕血液中存在的肽聚糖识别蛋白(PGRP).为了进一步研究该蛋白的结构和功能,采用了生物信息学方法对其理化性质、疏水性/亲水性、信号肽、二级结构、三级结构、亚细胞定位和GO功能注释等进行了分析.结果发现,肽聚糖识别蛋白是一种分泌类蛋白,可能具有信号肽.其主要分子功能为肽聚糖受体,在生物学进程中发挥的主要作用为肽聚糖代谢和先天性免疫应答.

Utilizing two-dimensional electrophoresis and mass spectrometry,peptidoglycan recognition protein（PGRP）was identified in the present study.In order to further analysis the structure and function of the protein,we empolyed bioinformatics methods to study its physical and chemical properties,hydrophobic/hydrophilic,signal peptide,secondary structure and tertiary structure,subcellular localization and functional annotations.Results showed that as a kind of secreted protein PGRP might have signal peptide and its molecular function was peptidoglycan receptor.In biological process ontology,it may play important role in peptidoglycan catabolic and innate immune response.