摘要
The formation of protein coronas on nanomaterial will significantly alter the surface properties of nanomaterial in biological systems and subsequently impact biological responses including signaling, cellular uptake, transport, and toxicity etc. It is of critical importance to understand the formation of protein coronas on the surface of nanomaterial. Analytical techniques, especially mass spectrometry-based proteomics methods, are playing a key role for the qualitative and quantitative analyses of protein coronas on nanomaterial. In this review, the proteomic approaches developed for the characterization of protein coronas on various nanomaterials are introduced with the emphasis on the mass spectrometry-based proteomic strategies.
The formation of protein coronas on nanomaterial will significantly alter the surface properties of nanomaterial in biological systems and subsequently impact biological responses including signaling, cellular uptake, transport, and toxicity etc. It is of critical importance to understand the formation of protein coronas on the surface of nanomaterial. Analytical techniques, espe- cially mass spectrometry-based proteomics methods, are playing a key role for the qualitative and quantitative analyses of pro- tein coronas on nanomaterial. In this review, the proteomic approaches developed for the characterization of protein coronas on various nanomaterials are introduced with the emphasis on the mass spectrometry-based proteomic strategies.
基金
supported by the National Natural Science Foundation of China(21175134,21375125)
the Creative Research Group Project of National Natural Science Foundation of China(21321064)
关键词
蛋白质组学
纳米材料
表面特性
电晕
料形
学分
生物系统
生物反应
protein corona, nanomaterial, proteomics, mass spectrometry, identification and quantification, gel electrophoresis
