大鲵皮肤黏液的抗氧化性及安全性评价研究

Study on the toxicity and antioxidant activity of mucus powder from salamander skin

为研究大鲵皮肤黏液的抗氧化性及急毒性安全性评价,利用邻苯三酚自氧化法和DPPH法,测定了大鲵黏液对超氧阴离子自由基(O-·2)及DPPH自由基的清除率;通过对小鼠灌胃饲养实验检测大鲵皮肤黏液的急毒性,并采用气质联用色谱分析黏液中的毒性成分。结果显示:大鲵黏液对超氧阴离子自由基的清除率为6.77%,对DPPH自由基的清除率为90.25%;大鲵黏液全粉的LD50值为4.64 g/kg,毒性成分为二甲基二硫醚(C2H6S2)。表明大鲵黏液具有抗氧化性和低毒性,经脱毒后可开发为保健产品。

The study focuses on antioxidant performance and safety evaluation of acute toxicity of salamander skin mucus. The study first uses pyrogallol autoxidation and DPPH method to determine salamander mucus to superoxide anion radical（ O-·2） scavenging and DPPH radical scavenging. Then it experiments with giant salamander breeding mice fed to test acute toxicity of Andrias davidianus skin mucus and analyses toxic constituents of mucus temperament by using GC-MS chromatography. The results show that the clearance rate of Salamander mucus flour to superoxide anion radical and to DPPH radical scavenging is 6. 77% and 90. 25%respectively,and that LD50 value of Andrias mucus powder is 4. 64 g / kg and toxic ingredient is imethyl disulfide（ C2H6S2）. It concludes that Andrias mucus is antioxidant and low in toxicity,and that it can be developed for health care products after detoxification.