摘要
[Objective]This study aimed to investigate various factors affecting the dedifferentiation of different floral organs of Jinxiu wild chive.[Method]Floral organs of Jinxiu wild chive were cultured in vitro,to analyze the effects of basic medium,exogenous hormone type and combination,and blossoming status of buds on callus induction.[Result]MS medium was the most suitable medium for callus induction from buds.NAA was conducive to improving callus formation rate compared with other auxins.Non-blossomed buds exhibited the highest rate of callus induction.Under the same culture conditions,the rate of callus induction varied extremely significantly among various floral organs;to be specific,the rate of callus induction from floral disc reached the highest,followed successively by bud and pedicel,but no callus was formed in floral axis group.[Conclusion]This study laid the foundation for further establishment of tissue culture and rapid propagation system of Jinxiu wild chive and other wild chives and provided technical support for the preservation,development and utilization of wild chive germplasm resources.
[Objective]This study aimed to investigate various factors affecting the dedifferentiation of different floral organs of Jinxiu wild chive.[Method]Floral organs of Jinxiu wild chive were cultured in vitro,to analyze the effects of basic medium,exogenous hormone type and combination,and blossoming status of buds on callus induction.[Result]MS medium was the most suitable medium for callus induction from buds.NAA was conducive to improving callus formation rate compared with other auxins.Non-blossomed buds exhibited the highest rate of callus induction.Under the same culture conditions,the rate of callus induction varied extremely significantly among various floral organs;to be specific,the rate of callus induction from floral disc reached the highest,followed successively by bud and pedicel,but no callus was formed in floral axis group.[Conclusion]This study laid the foundation for further establishment of tissue culture and rapid propagation system of Jinxiu wild chive and other wild chives and provided technical support for the preservation,development and utilization of wild chive germplasm resources.
基金
Supported by Special Fund for Bulk Vegetable Innovation Team of Guangxi Zhuang Autonomous Region from National Modern Agriculture Industry Technology System(nycytxgxcxtd-03-10-1)
Natural Science Foundation of Guangxi Zhuang Autonomous Region(2011GXNSFB018033)
Science and Technology Development Fund of Guangxi Academy of Agricultural Sciences(201001)
Key Project of Science and Technology of Guangxi Zhuang Autonomous Region(GKG 0323001-1)
Project of Nanning Bureau of Science and Technology(201102057B)
