玉米植株体内禾谷镰孢菌的PCR检测与症状对应表现分析

Analysis of Symptoms Associated with Fusarium graminearum Infections Detected by PCR in Maize

以高感玉米茎腐病自交系掖478为材料,用带禾谷镰孢菌牙签接种4叶期的玉米幼苗,7 d后调查接种植株,发现植株有枯萎症状和正常生长2种类型。通过特异引物对不同症状表现的植株进行PCR检测,结果表明,只有表现枯萎症状的植株体内能够检测到禾谷镰孢菌,苗期接种后仍正常生长的植株茎秆内无禾谷镰孢菌的存在。

Maize inbred line Ye478, high-sensitive to stalk rot, was inoculated with Fusarium graminearum.F. graminearum was cultivated using tooth picks and 4-leaf plants as the inoculation. Withered or normal plantswere divided after 7 days later. The results showed that F. graminearum were detected only in withered plants butnot in normal, and there was no F. graminearum in withered plants stalk when they had the inoculation in seedling,they can continue to grow normally.