超声波辅助提取多穗石柯根皮苷研究

Study on Ultrasonic Assisted Extraction Technology of Phloridzin in Lithocarpus polysachyus Rehd.

旨在优选超声波辅助提取多穗石柯根皮苷工艺。采用控制单一变量法,分别确定超声波辅助提取多穗石柯根皮苷的料液比、乙醇浓度、浸提温度、浸提时间较优范围,然后采用L9(34)正交实验及最优组合验证实验方案优化筛选最佳提取工艺。影响多穗石柯根皮苷提取得率的因素影响大小依次为:提取时间>乙醇浓度>提取温度>料液比,最佳提取工艺条件为提取温度45℃、料液比1:30、70%乙醇浓度、提取时间35 min,在此提取工艺条件下,多穗石柯根皮苷提取得率1.421%,提取物中根皮苷纯度为3.797%,验证性实验的相对偏差RSD=0.881%,说明该最佳提取工艺重复性和稳定性较好。

The aim was to prioritize the ultrasonic-assisted extraction technology of phloridzin in Lithocarpus polysachyus Rehd.. By controlling the change of mono factors, the optimal range of the solid- liquid ratio,ethanol concentration, extraction temperature and extraction time were validated with ultrasonic- assistedextraction in Lithocarpus polysachyus Rehd.. And then L9（3^4） orthogonal design and verification experimentswere used to determine the optimal extraction process. Factors influencing the extraction yield of phloridzin in Lithocarpus polystachyus were as follows： extraction time 〉ethanol concentration 〉extraction temperature〉 solid-liquid ratio. The optimal extraction conditions were as follows, extraction temperature 45℃, solid-liquidratio 1：30, extraction solvent 70% ethanol, and extraction time 35 min. Under these extraction processconditions, the extraction yield of phloridzin was 1.421% and the content of phloridzin was 3.797%. The RSD of verified experiment was 0.881%, which indicated that the optimal extraction process had goodreproducibility and stability.