HPLC法同时测定疏风解毒胶囊中7种活性成分

Simultaneous determination of seven active components in Shufeng Jiedu Capsule by HPLC

目的建立同时测定疏风解毒胶囊中戟叶马鞭草苷、马鞭草苷、虎杖苷、连翘酯苷A、毛蕊花糖苷、甘草酸单铵盐和大黄素的RP-HPLC方法。方法采用RP-HPLC法测定,色谱柱为Unitary C18柱（200 mm×4.6 mm,5μm）;流动相为乙腈-0.1%甲酸水溶液,梯度洗脱;体积流量1.0 m L/min;柱温30℃;检测波长250 nm;进样量10μL。结果戟叶马鞭草苷、马鞭草苷、虎杖苷、连翘酯苷A、毛蕊花糖苷、甘草酸单铵盐和大黄素分别在0.096~1.920、0.089~1.784、0.119~2.348、0.059~1.176、0.021~4.224、0.206~4.120、0.053~1.064μg与色谱峰面积呈良好的线性关系;平均加样回收率分别为98.43%（RSD 2.13%）、98.30%（RSD 2.68%）、99.31%（RSD 2.76%）、101.64%（RSD 2.37%）、97.47%（RSD 2.05%）、100.89%（RSD1.98%）、99.05%（RSD 2.21%）。结论该方法简单、快速、准确,为疏风解毒胶囊的质量控制提供一种可靠的参考方法。

Objective To establish a reverse phase high performance liquid chromatographic（RP-HPL） method for the simultaneous determination of the contents of hastatoside,verbenalin,polydatin,ofrsytlloside A,verbascoside,ammonium glycyrrhizinate,and emodin in Shufeng Jiedu Capsule.Methods RP-HPLC method was applied with the chromatographic condition as follows：the chromatographic column was Unitary C_（18） column（200 mm×4.6 mm,5μm）,acetonitrile-0.1%formic acid as the mobile phrase with gradient elution,the flow rate was 1.0 mL/min,the detection wavelength was 250 nm,the column temperature was 30 °C,and the injection volume was 10μL.Results Linearity relationships of hastatoside,verbenalin,polydatin,ofrsytlloside A,verbascoside,ammonium glycyrrhizinate,and emodin were 0.096-1.920,0.089-1.784,0.119-2.348,0.059-1.176,0.021-4.224,0.206-4.120,and 0.053-1.064μg,respectively,as well as the average recoveries of 98.43%（RSD 2.13%）,98.30%（RSD 2.68%）,99.31%（RSD 2.76%）,101.64%（RSD 2.37%）,97.47%（RSD 2.05%）,100.89%（RSD 1.98%）,and 99.05%（RSD 2.21%）.Conclusion The method is simple,fast,accurate,and can be used to control the quality of Shufeng Jiedu Capsule.