摘要
建立离子液体1-己基-3-甲基咪唑六氟磷酸盐([HMIM]PF_6)/甲醇溶液为萃取剂超声萃取,结合反相高效液相色谱同时测定山高粱中槲皮素-3-O-β-D-葡萄糖苷、山柰酚-3-β-D-葡萄糖苷、槲皮素和山柰酚4种黄酮成分的方法。采用Purospher star RP-C_(18)色谱柱(4.6 mm×250 mm,5μm)进行分离,以乙腈-甲醇-0.4%磷酸水溶液梯度洗脱,流速0.7 mL·min^(-1),紫外检测波长360 nm,柱温室温。在优化条件下,槲皮素-3-O-β-D-葡萄糖苷、山柰酚-3-β-D-葡萄糖苷、槲皮素和山柰酚分别在2.54×10^(-2)~2.54,2.50×10^(-2)~2.50,1.54×10^(-3)~0.154,1.49×10^(-3)~0.149μg线性关系良好,平均加样回收率分别为101.1%,98.90%,101.0%,101.6%。该方法绿色环保、简单快速,结果准确可靠,为山高粱中槲皮素-3-O-β-D-葡萄糖苷、山柰酚-3-β-D-葡萄糖苷、槲皮素和山柰酚的分析提供了有效的方法。
In order to established a method for simultaneous determination of isoquercitrin, astragaline, quercetin and kaempferol in Lysimachia clethroides, the ionic liquid 1-hexyl-3-methylimidazolium hexafluorophosphate ([HMIM]PF6)-methanol was used as the ultrasound-assisted extraction solvent combing with RP-HPLC. A Purospher star RP-C18 column was used with the mobile phase of acetonitrile, methanol and 0.4% phosphate acid by gradient elution at the detection wavelength of 360 nm. The flow rate was 0.7 mL·min^-1, and the column temperature was the room temperature. Under the optimized conditions, the linear ranges were 2.54×10^-2-2.54,2.50×10^-2-2.50,1.54×10^-3-0.154, 1.49×10^-3-0.149 μg for isoquercitrin, astragaline, quercetin and kaempferol, respectively. The average recoveries of the four constituents were 101.1%,98.90%,101.0%,101.6%, respectively. The method was green, simple, rapid and accurate, and provided a valid method for analysis of isoquercitrin, astragaline, quercetin and kaempferol in L. clethroides.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2015年第7期1305-1310,共6页
China Journal of Chinese Materia Medica
基金
河南省青年骨干教师计划项目(教高[2012]704号)
河南省科技厅基础前沿计划项目(132300410255
142300410123)
河南省教育厅自然科学研究项目计划项目(2010A210002)
河南大学研究生教育综合改革项目(Y1411008)
河南大学大学生创新创业训练计划项目(14NA016)
河南大学民生学院教育教学改革研究项目(MSJG2014052)
河南省高等学校重点科研项目(15A360014)