玫瑰树中非生物碱类化学成分研究

Studies on non-alkaloid constituents from Ochrosia borbonica

目的研究玫瑰树Ochrosia borbonica中的非生物碱类化学成分。方法采用硅胶、ODS、Sephadex LH-20柱色谱及制备HPLC等方法进行分离,采用理化分析及NMR、MS等谱学方法鉴定化合物结构。结果从玫瑰树枝叶90%乙醇提取物的石油醚萃取部位分离得到15个化合物,分别鉴定为羽扇豆醇(1)、羽扇豆酮(2)、白桦醇(3)、白桦酸(4)、熊果酸(5)、2α,3β-二羟基熊果酸(6)、11,12-去氢熊果酸内酯(7)、β-香树脂醇(8)、齐墩果内酯(9)、麦珠子酸(10)、木栓酮(11)、胆甾醇(12)、24R-乙基-5α-胆甾烷-3β,6α-二醇(13)、豆甾-7-烯-3β-醇(14)和β-谷甾醇(15)。结论所有化合物均为首次从玫瑰树属植物中分离得到。

Objective To study the non-alkaloid constituents from the stems and leaves of Ochrosia borbonica. Methods The chemical constituents were separated and purified by silica gel, Sephadex LH-20, ODS column chromatographies, and preparative HPLC. Their structures were determined by physicoehemical properties, spectral data, as well as comparison with the data in literature. Results Fifteen compounds were isolated from the petroleum ether fraction of 70% ethanol extract from the stems and leaves of O. borbonica, and identified as lupeol （1）, lupine ketone （2）, betulinic alcohol （3）, betulinic acid （4）, ursolic acid （5）, 2α,3β-dihydroxy-ursolic acid （6）, 11,12-dehydroursolic acid lactone （7）, β-amyrin （8）, oleanolic lactone （9）, alphitolic acid （10）, friedelin （11）, cholesterol （12）, 24R-ethyl-5α-cholestane-313, 6α-diol （13）, stigmast-7-en-3β-ol （14）, and β-sitosterol （15）. Conclusion All the compounds are isolated from the plants of Ochrosia Juss. for the first time.