UPLC结合化学计量学方法的肿节风指纹图谱研究

Fingerprint of Sarcandrae Herba by UPLC coupled with chemometrics methods

目的建立不同产地肿节风药材超高效液相色谱指纹图谱,为建立快速而高效的肿节风药材整体质量控制方法提供实验依据。方法采用UPLC法,色谱柱为Acquity HSS T3柱（50 mm×2.1 mm,1.8μm）,采用乙腈（D）-水（C）（各含0.1%甲酸）为流动相进行梯度洗脱,体积流量0.5 m L/min,检测波长330 nm,柱温35℃,建立肿节风药材指纹图谱;通过相似度评价,结合聚类分析和主成分分析（PCA）,对不同批次的肿节风药材进行质量评价。结果建立的肿节风药材UPLC指纹图谱方法确证16个共有峰,并对9个共有峰进行了明确化学成分指认;18批肿节风药材的相似度为0.869~0.979;通过聚类分析可大致聚成2类;PCA结果支持了聚类分析结果,并发现指纹图谱中迷迭香酸、新绿原酸、隐绿原酸等5个差异性较大的化合物。结论肿节风的UPLC指纹图谱的构建和化学模式的识别为药材质量控制提供更全面的参考。

Objective To establish the UPLC fingerprint for effective quality control ofSarcandrae Herba. Methods The fingerprint ofSarcandrae Herba was developed with ultra-performance liquid chromatography, and the Acquity UPLC HSS T3 column （50 mm × 2.1 mm, 1.8 μm） was used in the gradient elution with a mobile phase of acetonitrile-water （both containing 0.1% formic acid）： The flow rate was 0.5 mL/min, the colunm temperature was 35 ℃, and the detection wavelength was at 330 nm. Similarity evaluation combined with hierarchical clustering analysis （HCA） and prin.cipal components analysis （PCA） were used to evaluate the quality of herbs from different areas. Results The fingerprint of Sarcandrae Herba was established with good precision, reproducibility, and stability obtaining within 23 min, and nine peaks in the fingerprint were designed. Eighteen samples could be classified into two clusters. The PCA result was consistent with the HCA. A brief list of five differential compounds is presented, including rosmarinic acid, newchlorogenic acid, and so on. Conclusion The establishment of UPLC fingerprint ofSarcandrae Herba and the application of chemical pattern recognition can provide a more comprehensive reference for the quality control of herbs.