油茶根际高效溶磷细菌的筛选、鉴定及其安全性测试

Screening,Identification and Security Test of Camellia oleifera Rhizosphere Phosphate-Solubilizing Bacteria

本研究利用溶磷圈法和钼锑抗比色法,从油茶根际分离出20株高效溶无机磷细菌,并对其溶磷能力进行定性和定量测试,结果发现,D/d与溶磷细菌的有效磷含量之间没有显著的相关性,而培养液p H与有效磷含量之间存在极显著的负相关性关系(P<0.01)。并采用形态特征、生理生化、Biolog系统和16Sr DNA序列分析对一株溶磷效果最好的菌株NC285进行菌株鉴定,确定其为Bacillus aryabhattai。通过苜蓿植物模型和cbl A毒力基因测定对该菌株进行安全性检测,结果发现,该菌株未检测到cbl A基因,对洋葱和苜蓿安全无致病性。本研究对溶磷细菌的安全应用及提高油茶土壤磷素利用效率具有重要意义。

Twenty strains of inorganic phosphate-solubilizing bacteria were isolated from Camellia oleifera rhizosphere using phosphate-solubilizing circle and the molybdenum-anti-spectrophotometric method. The phosphate-solubilizing capacity was investigated from 20 strains based on qualitative and quantitative test. The results showed that there was a significant negative correlation between the phosphate solubilizing capacity and the pH medium（P 〈0. O1 ）, whereas there was no such phosphate solubilizing capacity on D/d. The strain NC285 was identified by its morpho- logical, physiological and biochemical characteristics, Biolog system and 16SrDNA gene sequence analysis. The high effective phosphate-solubilizing bacterium of NC285 was identified as Bacillus aryabhattai. The virulence of NC285 was tested on Allium cepa and Medicago sativa seedlings, and the cblA virulence genes. No cblA gene was detected on NC285 in the test, so this strain is safe for A. cepa and M. sativa.