唐古特白刺悬浮细胞对盐胁迫的生长与生理响应

Growth and Physiological Response of Nitraria tangutorum to Salt Stress

以唐古特白刺悬浮细胞为研究对象,对比分析了不同盐(Na Cl)浓度(0、100、150、200、250 mmol·L-1)胁迫下细胞生长状态和可溶性蛋白、可溶性糖、脯氨酸、过氧化物酶(POD)、超氧化物歧化酶(SOD)、丙二醛(MDA)等生理生化指标随培养时间的动态变化及其相关性。结果表明:(1)盐胁迫对白刺悬浮细胞生长状态影响明显,细胞生长符合Logistic生长曲线模型,其中,盐浓度100 mmol·L-1胁迫下白刺悬浮细胞生长速度最快,之后随着盐浓度的增加细胞生长逐渐受抑。(2)盐胁迫对白刺细胞生理生化指标影响明显,在培养初期,各指标随盐浓度的增加而增加,但随着培养时间的延长这种趋势逐渐减弱,其中,脯氨酸、SOD、MDA含量在培养过程中逐渐表现为随盐浓度的增加呈先增高后降低的趋势;在相同盐浓度胁迫下,渗透调节指标中,可溶性糖含量随培养时间的延长最先达到最大值,抗氧化酶中的SOD较POD更早达到最大值,分别是渗透调节和抗氧化酶调节的第一道防线。(3)白刺细胞鲜质量分别与可溶性糖、丙二醛含量呈极显著和显著负相关,各生理指标之间的相关性均达极显著正相关,表明盐胁迫下白刺细胞内生理生化的平衡是通过多种物质的协同作用来调节的。

Taking Nitraria tangutorum suspension cells as material and treated by different salt concentrations （0, 100, 150,200 and 250 mmol L-1 NaCl）, the growth characteristics of cells and the dynamic changes and correla- tions of physiological indexes with time, including soluble protein, soluble sugar, proline, peroxidase （POD） , su- peroxide dismutase （SOD） and malondialdehyde （MDA）, were analyzed. The results are as follows. （1）Salt treat- ment could significantly affect the growth of suspension cells, the cells growth conform to the Logistic growth curve model and the growth of suspension cells treated by 100 mmol ~ L-1 NaC1 was the fastest, and with the salt concen- tration increasing the growth were inhibited. （2） Salt treatment could significantly affect the physiological indexes, and at the early stages of culture all the indexes increased with the salt concentration increasing. While this trend decreased gradually with the extending of incubation time and the Proline, SOD, MDA increased in initial stage and then decreased with the salt concentration increasing in the later period of culture. Under same salt concentration, the soluble sugar got to the maximum at first in the osmotic adjustment index and the SOD got to the maximum in in- itial stage in the antioxidant enzymes index. And they are the first line of defensing the salt stress in osmotic adjustment and antioxidant enzymes respectively. （3） The fresh weight of suspension cells was significant and fearfully significant negatively correlated with the contents of soluble sugar and MDA. All the physiological indexes showed a significantly positive correlation. It indicated that the balance of cells internal physiological metabolic processes was regulated by various substances synergies under the salt environment.