地涌金莲组织培养中的褐化抑制

Studies on Anti-Browning During Tissue Culture of Musella lasiocarpa

地涌金莲组织培养中的褐化问题一直是其优良品种选育及规模化生产的技术瓶颈。为解决这一问题,以地涌金莲未成熟雄花为试材,研究了外植体消毒方式、植物生长调节剂种类及配比和不同抗褐化剂对愈伤组织褐化的影响。结果表明,未经消毒的外植体其污染率为0%,且褐化率和褐化指数均显著低于消毒后的外植体;6-BA在地涌金莲愈伤组织诱导中起主要作用,浓度为2 mg·L-1、3 mg·L-1时有利于愈伤组织的诱导,能显著降低褐化指数;添加柠檬酸+抗坏血酸(VC)混合液、表面添加VC均对地涌金莲褐化未起到明显抑制作用,姜汁和阿魏酸对抑制愈伤组织褐化有较好的效果,48.5 mg·L-1的阿魏酸能显著降低愈伤组织的褐化,使愈伤组织分化率达17.9%,较对照的2.9%提高了15%。

Callus necrosis during in vitro propagation is a large obstacles in breeding and large scale production of Musella lasiocarpa. To solve this problem, the effects of sterilizing methods of explants, the ratio of plant growth regulators and 4 kinds of browning inhibitors on callus browning were studied with the callus induced from its imma- ture male flowers as the explants. The results indicated that the contamination rate of unsterilized explants was 0%, and its browning rate and browning index were all significantly lower than that of the explants after disinfection; 6- BA played a major role in callus induction of M. lasiocarpa. It conduced to callus induction and significantly re- duced browning index when the concentration of 6-BA was 2 mg L-l or 3 mg L-1 Adding VC on the surface and VC mixture of citric acid failed to suppress browning. However, Ginger and ferulic acid gave satisfactory result to reduced browning, 48.5 mg L-1 ferulic acid could significantly reduce the callus browning and increased the rate of differentiation tolT. 9%, 15% higher than that of the control.