转化生长因子β1与乳腺癌微血管密度及预后的相关性

Correlation of Transforming Growth Factor β1 with Microvessel Density and Prognosis in Breast Cancer

目的:探讨乳腺癌组织中转化生长因子β1(transforming growth factorβ1,TGF-β1)与微血管密度(microvessel density,MVD)及乳腺癌预后的相关性。方法:采用免疫组织化学方法检测62例乳腺癌组织及30例乳腺腺瘤组织中TGF-β1的表达水平,计数MVD,分析二者与乳腺癌病理特征的关系。结合随访资料分析TGF-β1与乳腺癌的复发转移及患者生存率的相关性。结果:乳腺癌组织中TGF-β1蛋白表达的阳性率、MVD计数均明显高于乳腺腺瘤组织(P<0.05);乳腺癌组织中TGF-β1蛋白表达的阳性率随肿瘤直径的增加、临床分期增高及淋巴结转移而增高(P<0.05),且MVD计数与临床分期及淋巴结转移相关(P<0.05)。TGF-β1蛋白表达水平与乳腺癌组织MVD计数呈正相关(r=0.705,P<0.05)。术后5年,TGF-β1蛋白阳性表达患者的生存率明显低于阴性表达患者(P<0.05)。结论:TGF-β1在乳腺癌组织中呈过表达状态且与MVD计数呈正相关,在乳腺癌发生、发展过程中发挥重要作用,有望成为乳腺癌抗血管形成治疗的生物靶点。

Objective：To explore the correlation of the expression of transforming growth factorβ1（TGF-β1）in breast cancer tissues with the microvessel density（MVD）in breast cancer tissues,as well as the prognosis of breast cancer patients.Methods：The expression of TGF-β1in the tissues of breast cancer（n=62）and breast fibroadenoma（n=30）were detected with immunohistochemical staining,and the MVD was counted.The correlation of the two factors with the pathological features were analyzed.The influence of TGF-β1 on recurrence,metastasis and survival rate were analyzed in combination with the follow-up data.Results：The positive expression rate of TGF-β1 protein and the count of MVD in breast cancer tissues were significantly higher than those in breast fibroadenoma tissues（P〈0.05）.The positive expression rate of TGF-β1 protein in breast cancer tissues increased as the increase of tumor diameter,clinical stage,lymph node metastasis（P〈0.05）,and the count of MVD was correlated with clinical stage and lymph node metastasis（P〈0.05）.The expression level of TGF-β1 protein was positively correlated with the count of MVD in breast cancer tissues（r=0.705,P〈0.05）.The 5-year survival rate in patients with positive expression of TGF-β1 was significantly lower than those with negative expression of TGF-β1（P 0.05）.Conclusions：TGF-β1 is overexpressed in breast cancer tissues and is positively correlated with the count of MVD.TGF-β1plays an important role during the process of occurrence and development in breast cancer,and it is a potential biological target for anti-angiogenesis therapy in breast cancer.