摘要
目的:观察芪龙益肾汤对糖尿病肾病大鼠的治疗作用。方法:将60只SPF级Wistar雄性大鼠称重,随机选10只为正常对照组,以普通饲料喂养。剩余大鼠以高脂高糖饲料喂养8周后,空腹12 h,给予小剂量链脲佐菌素(剂量为30 mg·kg-1),腹腔注射,连续注射3 d。大鼠造模后称重,再随机将造模成功大鼠分为模型组、芪龙益肾汤高、中、低剂量组(剂量分别为每天25.2、12.6、6.3 g·kg-1)、厄贝沙坦组,每组9只。给药干预治疗8周后,将各组大鼠置于代谢笼中,收集24 h尿量,测定尿微量白蛋白(umAlb)。麻醉取血,分离血清,测定胱抑素C(Cys-c)、糖化血红蛋白(HbAlc)、空腹血糖(FPG)水平。结果:给药8周后,与正常对照组比较,模型组大鼠血清Cys-c、HbAlc、FPG及umAlb水平明显升高,差异有统计学意义(P<0.05);与模型组比较,芪龙益肾汤中、高剂量组及厄贝沙坦组大鼠血清Cys-c、HbAlc、FPG及umAlb水平明显降低,差异有统计学意义(P<0.05)。结论:芪龙益肾汤能降低早期糖尿病肾病大鼠的血清Cys-c、HbAlc、FPG及umAlb表达水平,改善肾功能。
Objective： To observe the therapautic effects of Qilongyishen tang on rats with nephropathy （DN）. Methods： 60 Wistar SPF rats were weighed, 10 rats were randomly selected as normal control group and fed them with ordinary diet. While the other rats were fed with high fat diet for 8 weeks then injected with streptozotocin（STZ） in lower dose of 30 mg/kg for three days after fasting for 12 hours. When the diabetic nephropathy model was establishied, rats were weighed and divided randomly into model group, group treated with Irbesantan and groups treated with traditional Chinese medicine in low, medium and high dose. Each group had 9 rats. All rats were placed in metabolic cages and observed the general vital energy, urinary production and 24-hour urine preotein after consecutive 8 weeks intervention. The rats were then anesthetized and blood samples were collected. Finally, the serum was separated and the level of Cys-c and HbAlc was determined. Result： The level of Cys-e, HbAlc, FPG and umAlb in the model group were singnificantly higher than that in the normal group（ P 〈 0. 05 ） , which were in the treatment with traditional Chinese medicine in medium and high dose and Irbesantan group were significantly lower than those that were in the model group（P 〈0. 05）. Conclusion： Qilongyishen tang can reduce the level of early diabetic nephropathy rats＇ serum Cys-c, HbAlc, FPG and umAlb.
出处
《中国现代中药》
CAS
2015年第4期341-344,共4页
Modern Chinese Medicine
基金
甘肃省中医药管理局基金资助项目(GZK-2012-63)
