楤木皂苷对大鼠心肌缺血/再灌注损伤的保护作用

Protective Effects of Total Saponins Extracted from Aralia Taibaiensis on Myocardial Ischemia/Reperfusion Injury

目的:观察楤木皂苷(total saponins extracted from Aralia taibaiensis,s AT)对大鼠心肌缺血/再灌注(myocardia1 ischemia/reperfusion,MI/R)损伤的影响。方法:可逆性冠脉左前降支结扎缺血30 min再灌注3 h复制MI/R模型,将SD大鼠随机分为假手术组、模型组、s AT低、中、高剂量组,每组10只。采用伊文思蓝(EB)、2,3,5-氯化三苯基四氮唑蓝(TTC)双染法测定心肌梗死面积,苏木精-伊红(HE)染色法观察心肌病理学形态变化,并检测血清中乳酸脱氢酶(LDH)、肌酸激酶同工酶(CK-MB)、超氧化物歧化酶(SOD)、丙二醛(MDA)、过氧化氢酶(CAT)及谷胱甘肽过氧化物酶(GSH-Px)水平。结果:与模型组比较,s AT中、高剂量组可明显缩小心肌梗死面积(P<0.05),并显著降低血清中LDH、CK-MB及MDA的含量,同时使得血清中SOD、CAT和GSH-Px的活性增加。且所有给药组心肌组织的病理损伤也小于模型组。结论:s AT对大鼠MI/R损伤具有保护作用,其机制可能与抗氧化作用相关。

Objective： To investigate the protective effects of total saponins extracted from Aralia taibaiensis （sAT） on myocardial ischemia/reperfusion injury in rats. Methods： An in vivo model of myocardial ischemia 30 min followed by 3 h reperfusion was made by reversibly ligating coronary left descending artery. The male SD rats were divided randomly into sham group; ischemia/reperfusion model group; sAT （60 mg·kg^-1; 120 mg·kg^-1; 240 mg·kg^-1） groups （n=10）. The area of myocardial infarction and the change of histological tissue were assessed after 3 h reperfusion. Meanwhile, the levels of lactate dehydrogenase （LDH）, creatine kinase-MB （CK-MB）, superoxide dismutase （SOD）, Malondialdehyde （MDA）, eatalase （CAT） and glu^thione peroxidase （GSH-Px） in serum were determined by assay kits. Results： Compared with model group, pretreatment with sAT significantly reduced infarct size, decreased the levels of LDH, CK-MB and MDA in the serum, and increased the activities of SOD, CAT and GSH-Px. In addition, sAT improved the pathologic changes of myocardium. Conclusion： sAT has cardioprotective effect against MI/R injury. The mechanism may be related to its antioxidant activities.