急性肺损伤谷胱甘肽的实验研究

An Approach to Glutathione Redox Cycle in Experimental Acute Lung Injury

用家兔经右心插管注入油酸制成急性肺损伤模型，观察谷胱甘肽（ＧＳＨ）含量、谷胱甘肽过氧化物酶（ＧＳＨｐｘ）、过氧化氢酶（ＣＡＴ）活力及丙二醛（ＭＤＡ）含量。结果显示，损伤组血液，肺泡灌洗液（ＢＡＬＦ）、肺匀浆中ＭＤＡ含量较对照组升高，血液及ＢＡＬＦ中，ＣＡＴ、ＧＳＨｐｘ活力提高。ＧＳＨ在对照组ＢＡＬＦ中含量较高，还原型ＧＳＨ占９１．３％，而损伤组ＢＡＬＦ中ＧＳＨ含量明显下降（Ｐ＜０．０１），且氧化型ＧＳＨ（ＧＳＳＧ）含量增加（３２．６％），ＧＳＨ下降与ＭＤＡ升高呈负相关。提示，ＡＬＩ时氧化应激增强，ＧＳＨ在肺泡上皮衬液（ＥＬＦ）中含量降低，可能引起其氧化—抗氧化系统失衡从而增加肺泡上皮细胞对氧化损伤的易感性。

he contents of GSH,vitality of GSHpx and CAT and the contents of MDA were observed in acutelunginjury models of rabbits induced by oleic acids through right heart catheter. The results showed that MDA contents in blood,BALF and lung tissue homogenate of the injured group were higher than those of the control group .The vitality of CAT and GSHpx in blood and BALF increased.The content of GSH in BALF of the control group increased,with 91.3% in reduced form but that of the injured group decreased evidently (P<0.01) with the oxidized form GSH(GSSG) increased (32.6%). The decrease of GSH was negatively correlated with the increase of MDA. It is suggested that in acute lung injury the strengthened oxidative stress and the lowered content of GSH in alveolar lining fluid may induce imbalance of oxidant antioxidant system and therefore inerease the susceptibility of alveolar epithelium to oxidantmediated injury.