马铃薯苗期抗寒能力直接评价体系的建立与应用

Establishment and Application of Direct Cold-resistance Evaluation System for Potato Seedlings

通过不同温度处理马铃薯苗期植株,以评价叶片损伤程度为标准,建立马铃薯苗期抗寒能力的直接评价体系,并利用该评价体系和电解质渗漏法,对40份马铃薯材料进行抗寒能力测定和相关分析。结果显示,两种方法间具有极显著的直线相关关系(Y=8.65+1.75X,r=0.86,P<0.01),说明苗期抗寒能力直接评价体系可准确高效地评价马铃薯抗寒能力;同时也筛选获得了21份具有较好抗寒能力的马铃薯材料,为后期马铃薯抗寒相关研究和抗寒育种提供了材料保障。

Direct cold-resistance evaluation system for potato seedlings was established on the experiment of the potato seedlings treated with graded temperature in this research, in which the injury content of the leaves was used as standard. Meanwhile, the electrolyte leakage was used to validate this method. The results of correlation analysis of the direct cold-resistance evaluation system and electrolyte leakage, based on 40 potato varieties, showed that the system was effective and precise （Y=8.65 ＋ 1.75Z, r = 0.86, P〈 0.01 ）. Meanwhile, 21 varieties were confirmed with cold resistance and would be used in the further breeding for cold resistance.