马铃薯黑痣病菌生物学特性测定

Characterization of Biological Properties of Pathogen of Potato Black Scurf

马铃薯黑痣病是土传真菌性病害,在定西市马铃薯种植区均有发生,目前已成为影响马铃薯产量和品质的主要因素之一。本研究对马铃薯黑痣病菌从温度、光照、碳源、氮源以及p H方面进行了生物学特性的测定。结果表明:该病原菌菌丝在无光25℃条件下生长最快,在无光35℃条件下生长最慢;室温条件下培养1 d后用紫外线照射处理2 h,然后室温持续光照培养4 d的菌丝生长速率最大,持续黑暗培养4 d的菌丝生长速率最慢;不同碳氮源对该菌菌丝生长均有影响,碳源为淀粉的培养基上菌丝生长最快、氮源为尿素的培养基上菌丝生长最快;培养基p H中性时菌丝生长速率最大。

The potato black scurf is a soil-borne fungal disease, which happens in all of the areas of Dingxi. It is one of the main factors influencing potato quantity and quality. This research was to characterize biological properties of the pathogen causing potato black scurf in terms of temperature, illumination, carbon source, nitrogen source and pH value. The mycelium grew the fastest at 25℃ and the slowest at 35℃ under dark conditions. When cultured for 1 d at 25℃ , treated with UV for 2 h, and then kept under light for 4 d at room temperature, the mycelium grew the fastest, while the mycelium grew the slowest when kept under darkness for 4 d. The mycelium growth was affected by different sources of carbon and nitrogen. Starch was the best carbon source and urea was the best nitrogen source for the mycelium growth. The mycelium grew fastest when pH was set at 7.0.