摘要
病毒病是制约马铃薯产量和质量的重要因素。马铃薯脱毒试管苗、微型薯是生产脱毒种薯的重要环节。本研究于2010—2013年对收集自云南省的试管苗274个、微型薯356个，共计630个样品。应用电子显微镜观察，并采用DAS—ELISA检测了8种病毒：马铃薯s病毒、马铃薯卷叶病毒、马铃薯Y病毒、马铃薯x病毒、马铃薯A病毒、马铃薯M病毒、番茄斑萎病毒和烟草环斑病毒，发现试管苗和微型薯中马铃薯s病毒检出率最高（21．27％），其次是PLRV（5．71％）；还检测到了新出现的侵染马铃薯的番茄斑萎病毒和烟草环斑病毒。研究结果为马铃薯种薯生产过程中病毒病的检测防控提供了参考。
The viral diseases are important constrains to yield and quality of potato. In vitro plantlets and minitubers are the key steps in the production of virus-free seed potato. In this research, 630 samples including 274 in vitro plantlets and 356 minitubers were collected from 2010 to 2013 in Yunnan Province. The samples were detected through the electron microscopy. DAS-ELISAwas used to test PVS, PLRV, PVY, PVX, PVA, PVM, TSWV and TRSV. The results showed that PVS and PLRV were two predominant viruses, and 21.27% and 5.71% of the samples were infected by PVS and PLRV, respectively. TSWV and TRSV were two emerging viruses infecting potato in China. The results could provide a consult for detection and control of potato virus diseases in potato production.
出处
《中国马铃薯》
2015年第1期42-45,共4页
Chinese Potato Journal
基金
云南省现代农业马铃薯产业技术体系建设(2013KJTX003)
云南省农业科学院现代农业科研所及优秀团队建设(2014CZYY021)
云南省高层次科技人才培引工程(2011CI134)
关键词
试管苗
微型薯
脱毒种薯
主要病毒
检出率
分析
in vitro plantlet
minituber
virus-free seed potato
major virus
incidence
analysis
