骨髓单个核细胞与骨髓间充质干细胞促进大鼠皮瓣存活率的比较

Comparison between Bone Marrow-derived Mononuclear Cells and Mesenchymal Stem Cells in Promoting Survival Rates of Random-pattern Skin Flap in Rats

目的比较来源于等量骨髓的单个核细胞与经扩增的间充质干细胞,促进大鼠随意皮瓣成活率的效果。方法取等质等量的大鼠骨髓,一半直接离心获得骨髓单个核细胞,一半经体外培养获得骨髓间充质干细胞,注射相等数量(n=6)的大鼠随意皮瓣。术后测量并计算皮瓣成活面积,取材,行组织学检测,计数CD31阳性血管数量。结果未经培养的骨髓单个核细胞组皮瓣的平均存活率为(71.6±8.4)%,培养的骨髓间充质干细胞组平均存活率为(66.2±3.1)%,这两组存活率均显著高于注射平衡液的对照组(55.9±3.4)%;注射细胞组之间平均存活率没有统计学差异。组织学血管密度计数显示,骨髓单个核细胞组和骨髓间充质干细胞组的微血管数量分别是(58.2±6.8)和(42.7±5.1),都显著高于PBS对照组(22.8±3.1),而骨髓单个核细胞组也显著高于骨髓间充质干细胞组。结论与不经培养的骨髓单个核细胞相比,通过体外扩增的骨髓间充质细胞,未能显著提高大鼠随意皮瓣的成活率。

Objective To compare the non-cultured bone marrow mononuclear cells（BM-MNCs） and cultured bone marrow mesenchymal stem cells（BMSCs） in the promotion of random-pattern skin flap survival of rats. Methods BM-MNCs and BMSCs derived from two identical rat bone marrow aspirates were injected in a random-pattern skin flap model of rat（n=6）. The survival area was determined by its appearance, color and texture. Capillary density of flaps was measured by histology. Results The flap survival rates were（71.6±8.4）% in the BM-MNC-treated group and（66.2±3.1）% in the BMSCtreated group, both of which were significantly higher than the control group（55.9 ±3.4）%. However, no significant difference was observed between the cell transplanted groups. According to vessel density assay, capillary density in the BM-MNC-treated group（58.2±6.8） was higher than in the BMSC-treated group（42.7±5.1）, both of which were significantly higher than the control group（22.8±3.1）. Conclusion Pre-culture of BMSCs does not bring therapeutic benefits.