藏药牦牛角有效成分的分离与解热镇痛物质基础的研究

Separation of the Effective Components from Tibetan Yak Horn and Its Antipyretic and Analgesic Activity

目的:寻找藏药材牦牛角中解热镇痛物质。方法:实验以牦牛角为原料,经回流提取得到粗提液,再经过透析和非变性电泳分离,得到不同分子量大小的多肽;采用皮下酵母注射法建立小鼠发热模型,热板法评价镇痛活性。结果:牦牛角回流粗提取液经透析、非变性电泳分离后,得到的小分子多肽在给药30min后能显著降低肛温,达到最大痛阈,并提高值到67%。结论:研究证实牦牛角粗提取液有明显的解热和镇痛作用,为进一步获得新的多肽类解热镇痛候选药物奠定基础。

Objective：Tibetan yak horns were selected to find the antipyretic and analgesic substances.Methods：As raw material, the yak horns were grinded to obtain a crude extract by reflux extraction, then they were treated by dialysis and non-denaturing gel electrophoresis separation, and the different molecular weight peptides were obtained;by using sub-cutaneous injection of yeast,the mouse fever model was established, and the hot plate method was established to evaluate the analgesic activity.Results：The small molecular peptides could significantly reduce rectal temperature, and get max-imum pain threshold, and the value was increased to 67%.Conclusion： Our study confirmed the crude extracts from horns have significantly antipyretic and analgesic effects, and our finding is important for further getting a new class of anti-inflammatory candidate peptide drugs.