摘要
目的:寻找藏药材牦牛角中解热镇痛物质。方法:实验以牦牛角为原料,经回流提取得到粗提液,再经过透析和非变性电泳分离,得到不同分子量大小的多肽;采用皮下酵母注射法建立小鼠发热模型,热板法评价镇痛活性。结果:牦牛角回流粗提取液经透析、非变性电泳分离后,得到的小分子多肽在给药30min后能显著降低肛温,达到最大痛阈,并提高值到67%。结论:研究证实牦牛角粗提取液有明显的解热和镇痛作用,为进一步获得新的多肽类解热镇痛候选药物奠定基础。
Objective:Tibetan yak horns were selected to find the antipyretic and analgesic substances.Methods:As raw material, the yak horns were grinded to obtain a crude extract by reflux extraction, then they were treated by dialysis and non-denaturing gel electrophoresis separation, and the different molecular weight peptides were obtained;by using sub-cutaneous injection of yeast,the mouse fever model was established, and the hot plate method was established to evaluate the analgesic activity.Results:The small molecular peptides could significantly reduce rectal temperature, and get max-imum pain threshold, and the value was increased to 67%.Conclusion: Our study confirmed the crude extracts from horns have significantly antipyretic and analgesic effects, and our finding is important for further getting a new class of anti-inflammatory candidate peptide drugs.
出处
《中医药学报》
CAS
2015年第2期49-52,共4页
Acta Chinese Medicine and Pharmacology
基金
甘肃省牦牛繁育工程重点实验室开放课题资助
关键词
牦牛角
分离
凝胶电泳
小分子多肽
解热镇痛
Yak horn
Separation
Gel electrophoresis
Small molecular peptides
Antipyretic and analgesic function