大量维生素C辅助金纳多对小鼠急性肺损伤的保护作用

Protective effects of vitamin C combined with Ginaton on acute lung injury in mice

目的:探讨大量维生素C辅助银杏叶提取物金纳多对实验性急性肺损伤的保护作用。方法:将小鼠随机分为对照组,脂多糖(LPS)组,金纳多组,(金纳多+维生素C)组。采用测量肺组织湿/干重比(W/D)反映肺组织含水量的变化,支气管肺泡灌洗液(BALF)蛋白漏出量反映呼吸膜通透性的变化,用自由基代谢产物丙二醛(malondialdehyde,MDA)反映急性肺损伤时自由基水平。结果:(1)与对照组比较,LPS组、金纳多组、(金纳多+维生素C)组小鼠肺脏W/D值均明显升高(P<0.01);与LPS组比较,金纳多组、(金纳多+维生素C)组W/D值均明显降低(P<0.01)。(2)与对照组比较,LPS组、金纳多组、(金纳多+维生素C)组BALF蛋白浓度和MDA含量均明显升高(P<0.01);与LPS组比较,金纳多组、(金纳多+维生素C)组BALF蛋白浓度和MDA含量均明显降低(P<0.01);与金纳多组比较,(金纳多+维生素C)组BALF蛋白浓度和MDA含量均明显降低(P<0.01)。结论:银杏叶提取物和维生素C两种药物联合使用对急性实验性肺损伤具有保护作用,疗效满意,经济实用,可降低医疗成本和患者经济负担。

ObjectiveTo investigate the effects of vitamin C combined with Ginaton （ ginkgo biloba extract） on acute lung injury of mice. Methods ：The mice were randomly divided into the control group , the LPS group, and the Ginaton group （ Ginaton ＋vitamin C ） .The changes of lung water were determined by the wet /dry （ W/D） weight ratio of lung tissue ;the changes of respiratory membrane permeability were reflected by the protein leakage in bronchoalveolar lavage fluid （BALF）; the level of free radicals in acute lung injury was determined by malondialdehyde （MDA） metablized from free radicals.Results：（1）.Compared with the control group, the wet /dry （W/D） weight ratio of lung tissue decreased significantly in the LPS group, and the Ginaton group （ Ginaton ＋vitamin C ）（ P 〈0.01）.（2）;compared with the control group, protein con-centration and MDA contents increased in the LPS group and the Ginaton group （ Ginaton ＋vitamin C ）（ P 〈0.01）; compared with the LPS group, BALF protein concentration and MDA contents decreased significantly in the Ginaton group （ Ginaton ＋vitamin C ） （ P 〈0.01）. Conclusion：The combination of ginkgo biloba extract and vitamin C has a protective effect on experimental acute lung injury with a satisfactory ther-apeutic effect , which is economic , reducing the cost of medical care and the patients＆#39;economic burden.