知母皂甙元对原代培养神经细胞M受体刺激后cGMP生成速率的影响

The Effect of ZMS (A sapogenin from Zhimu, Rhizoma Anemarrhenae) on Carbachol-Induced cGMP Production in Primary Neuronal Culture

目的：观察知母皂甙元（ＺＭＳ）对原代培养神经细胞Ｍ受体刺激后ｃＧＭＰ生成速率的影响。方法：用放射免疫分析法测定细胞内ｃＧＭＰ水平，观察ＺＭＳ不同作用时间对Ｃａｒｂａｃｈｏｌ刺激下ｃＧＭＰ生成的影响。结果：ＺＭＳ短时间（５ｍｉｎ）作用于原代培养的神经细胞对Ｃａｒｂａｃｈｏｌ刺激下ｃＧＭＰ的生成无影响，而长时间（４８ｈ）作用明显提高Ｃａｒｂａｃｈｏｌ的刺激下ｃＧＭＰ生成量。结论：ＺＭＳ的作用与激动剂或拮抗剂不同，需要较长时间的作用才能发挥作用。

Intracellular cyclic GMP(cGMP) levels were measured by radioimmunoassay, and the effects of a sapogenin from Zhimu (ZMS) on cGMP formation stimulated by carbachol were investigated. The result showed that carbachol stimulated cGMP formation was not affected by acute exposure but was markedly elevated by chronic exposure to ZMS of primarily cultured neurons.