摘要
目的研究"益肾调督"针灸法对阿尔茨海默病(AD)模型大鼠海马CA1区神经元轴突生长导向因子导素(Netrins)表达的影响。方法将Wistar大鼠按随机数字表分为4组,即正常组、假手术组、模型组和针灸治疗组,采用双侧海马注射Aβ1-42的模型复制方法,针灸治疗组采用先针刺再艾灸"百会"和"肾俞"穴,通过免疫荧光法观察大鼠海马CA1区Netrins的表达变化。结果模型组大鼠海马CA1区Netrins积分光密度与正常组相比显著降低(P<0.01)。针灸治疗组大鼠海马CA1区Netrins积分光密度与模型组相比明显升高(P<0.01)。结论针灸可能通过增加Netrins的表达,促进轴突再生,进而起到防治AD的作用。
Abst racts:Objective To observe the effet of Yishentiaodu acupuncture -moxibustion on hippocampal CA1 area of Netrins in Alzheimer's disease rat,and to investigate the specific mechanism of preventive -treatment on AD by acupuncture -moxibustion.Methods Wistar rats were randomly divided into 4 groups: the normal group, sham -operated group, model group, and acupuncture -moxibustion group.First, bilateral hippocampus were injected Aβ1 -42 to make rat model of AD. Then, acupuncture -moxibustion group selected Baihui points and Shenshu points, and treated with acupuncture and moxibustion.Last, detect the expression of Netrins in rats by the method of Immunofluores-cence.Results Compared with normal group and sham -operated group,Neterins expression of model hippocampus was lower obviously than that of them(P 〈0.01).However, compared with the model group, Netrins expression of anupuncture -moxibustion group’ hippocam-pus CA1 area increased significantly(P 〈0.01).Conclusion Acupuncture -moxibustion can effectively improve of the expression of the Netrins, induce the axonal injured and promote axon regeneration, Which have the effect of prevention and treatment of AD.
出处
《湖北中医药大学学报》
2015年第2期10-12,共3页
Journal of Hubei University of Chinese Medicine
基金
国家自然基金项目(81273864
81173323)
关键词
阿尔茨海默病
益肾调督针灸法
百会
肾俞
导素
Yishentiodu Acupuncture -Moxibustion
Alzheimer's disease
Baihui
Shenshu
Netrins