木薯细菌性枯萎病病原菌的分离与鉴定

Isolation and Identification of Cassava Bacterial Blight Pathogen

采用组织分离法,从发病的木薯叶片上分离纯化病原菌,对纯化的病原菌进行菌落形态、致病性及生化特性的测定,并采用16S r DNA基因序列进行了分子鉴定和进化分析。结果表明,分离的木薯致病菌为黄单胞菌(Xanthomonas sp.)。

Cassava bacterial blight （CBB） is a destructive disease of cassava （Manihot esculenta） and is caused by Xanthomonas axonopodis pv manihotis,Xam. This disease had invoked a serious damage to cassava industry in other countries. The pathogen of the CBB was isolated from the infected leaves collected from cassava varieties SC8 and SC5 in Qiongzhong County, Hainan, and then purified for morphological observation, pathogenecity assay and biochemical assay. This pathogen was also identified by using 16S rDNA gene sequence analysis. The results showed that the isolated pathogen was Xanthomonas axonopodis.