摘要
目的探讨番茄红素对大鼠脊髓损伤后白细胞介素-6(IL-6)表达的影响及对神经功能恢复的作用。方法健康成年Sprague-Dawley大鼠36只,采用Allen法(10 g×25 mm)在T9造成大鼠脊髓损伤模型,并随机分为对照组(A组)、甲泼尼龙(MP)治疗组(B组)和番茄红素治疗组(C组),每组12只。造模成功30 min后,A组不给予治疗,B组腹腔注射MP 30 mg/kg,C组灌胃番茄红素20 mg/kg。于术后1 d、3 d、7 d分别对各组进行斜板试验。损伤段脊髓切片行免疫组织化学染色检测IL-6的表达。结果术后1 d和7 d,C组斜板试验评分均高于A组(P<0.05);术后1 d、3 d和7 d,B组斜板试验评分均高于A组(P<0.05)。术后1 d、3 d和7d,B组和C组IL-6表达均显著低于A组(P<0.001)。结论番茄红素可以通过抑制IL-6的表达,减轻急性脊髓损伤后的炎症反应,并促进大鼠神经和运动功能恢复。
Objective To explore the effects of lycopene on interleukin-6（IL-6） expression and motor function after spinal cord injury in rats. Methods 36 healthy adult Sprague-Dawley rats were randomly divided into control group（A）, methylprednisolone sodium succinate（MP） treatment group（B） and lycopene treatment group（C） with 12 rats in each group, and spinal cord injury model at T9 was established with modified Allen＇s technique（10 g×25 mm）. 30 minutes after modeling, group A received no treatment, group B was injected MP 30 mg/kg, group C was given lycopene 20 mg/kg. They were tested with inclined plate test 1 day, 3 days and 7 days after injury. The expression of IL-6 was examined with immunohistochemistry, then. Results The inclined plate test scores were higher in the group C than in the group A1 day and 7 days after injury（P〈0.05）, and in the group B than in the group A 1 day, 3 days and 7 days after injury（P〈0.05）. The expression of IL-6 was significantly lower in the groups B and C than in the group A 1 day, 3 days, 7 days after injury（P〈0.001）. Conclusion Lycopene can inhibit the expression of IL-6 in acute spinal cord injury to reduce the inflammation and facilitate the recovery of nerve and motor function.
出处
《中国康复理论与实践》
CSCD
北大核心
2015年第4期402-405,共4页
Chinese Journal of Rehabilitation Theory and Practice
