丙戊酸钠协同硼替佐米对RPMI8226细胞的促凋亡作用

Pro-apoptotic Effect of Valproic Acid and Bortezomib on RPMI8226 Cells

目的:以骨髓瘤细胞株RPMI8226为实验对象,观察丙戊酸钠(valproic acid,VPA)和硼替佐米(bortezomib,BZ)对此细胞株的增殖及凋亡的诱导情况。方法:实验分组如下:对照组,VPA单药组(1.0 mmol/L),BZ单药A组(10.0 nmol/L),BZ单药B组(20.0 nmol/L),BZ单药C组(35.0 nmol/L),联合用药A组(VPA1.0 mmol/L+BZ10.0 nmol/L),联合用药B组(VPA 1.0 mmol/L+BZ20.0 nmol/L),联合用药C组(VPA 1.0 mmol/L+BZ 35.0 nmol/L)。用MTT技术检测细胞增殖抑制情况;流式细胞仪检测凋亡比例。结果:丙戊酸钠与硼替佐米单用对RPMI8226细胞株细胞增殖有抑制作用,有细胞凋亡,但丙戊酸钠与硼替佐米协同用药A组、B组、C组增殖抑制可达75.1%及凋亡情况可达68.9%(P<0.01)。结论:丙戊酸钠与硼替佐米协同用药后对RPMI8226细胞增殖抑制及诱导凋亡作用更显著,丙戊酸钠对硼替佐米有增敏作用。

Objective： To investigate the effects of valproic acid（VPA） and bortezomib（BZ） on cell proliferation and apoptosis in RPMI8226 cells. Methods： The drug groups were set as follows： control group; VPA alone group （1.0 mmol/L）; BZ alone group A （10.0 nmol/L）; BZ alone group B （20.0 nmol/L）; BZ alone group C （35.0 nmol/L）; combination group A （VPA 1.0 mmol/L＋BZ 10.0 nmol/L）; combination group B （VPA 1.0 mmol/L＋BZ 20.0 nmol/L）; combination group C （VPA 1.0 mmol/L＋BZ 35.0 nmol/L）. The cell proliferation was tested by MTT and the apoptotic ratio was observed by flow cytometry. Results： Both valproic and bortezomib alone can inhibit the cell proliferation and induce the apoptosis in RPMI8226 cells; the cell death ratio was increased~ by 75.1%, and the apoptotic ratio was increased by 68.9%; when the cells were treated by valproic and bortezomib together, the effect was more obvious. Conclusions： The effect of valproic and bortezomib used together was significantly enhanced on promoting cell apoptosis and inhibiting the cell proliferation of RPMI8226 cells, suggesting that valproic plays a role.