维药异常黑胆质成熟剂总黄酮杀伤K562/ADR细胞株作用研究

Preliminary study on destruction K562/ADR cell effects of Abnormal Savda Munziq total flavonoids

目的探讨维药异常黑胆质成熟剂总黄酮（ASMq）对人慢性骨髓性白血病细胞（K562）及人白血病阿霉素耐药株（K562/ADR）的细胞毒性作用。方法对K562细胞株及K562/ADR细胞株细胞进行培养,在培养过程中注意K562/ADR细胞株对阿霉素的耐药性。取对数生长期K562、K562/ADR细胞株,96板孔中加入RPMI-1640培养液配制5×104个/m L细胞悬液100μL/每孔,37℃、5%CO2培养24 h贴壁,然后加入不同浓度（0、50、100、200、400、800和1600μg/m L）的ASMq,在37℃共培养48 h,每组5个重复,按照CCK-8说明书检测细胞活性。结果 K562细胞株对不同浓度的ASMq的细胞毒性的敏感性不同,差异有统计学意义（P〈0.05）;K562/ADR细胞株对0~1 600μg/m L浓度的ASMq的细胞毒性的敏感性差异无统计学意义（P〉0.05）;结论 ASMq对K562细胞具有细胞毒性作用,为临床治疗血液肿瘤提供了新的线索。

Objective To analyse cytotoxicity function of the abnormal savda munziq total flavonoids （ASMq）on K562 and K562/ADR of human being.Methods K562 and K562/ADR cells were developed to pay attention to adriamycin resistance of K562/ADR.The logarithmic phase K562 and K562/ADR cells 96-well plates with RPMI-1640 culture make up 5＆#215;10 4/mL cells suspension 100 μL/per hole,37℃ and 5%CO2 24 h post wall were pouring into different concentrations （0, 50, 100, 200, 400,800 and 1 600 μg/mL）of 37℃ ASMq trained 48 h to detect cell activity by the instruction （CCK-8）.Result There is a statistically significant difference （P ＆lt;0.05）of K562 with different concentration of ASMq cytotoxic sensitivity.The cytotoxicity of different concentration ASMq to K562/ADR has no statistical significance （P ＆gt;0.05）.Conclusion ASMq has cytotoxic effect on the K562 cells,Blood tumor chemotherapy to re-duce drug resistance for clinical treatment provides a new clue.