高效液相色谱法测定天香丹颗粒中红景天苷和丹参酮ⅡA的含量

Determination of Salidroside and Tanshinone Ⅱ_A in Tianxiangdan Granule by HPLC

目的建立测定天香丹颗粒中红景天苷和丹参酮ⅡA含量的高效液相色谱法。方法色谱条件：色谱柱为Hypersil ODS2（4.6 mm×250 mm,5μm）;乙腈-0.03%磷酸水溶液（7：93）作为流动相;检测波长220 nm;流速为1.0 m L/min。结果红景天苷在5.44~16.32μg范围内与峰面积呈良好的线性关系（r=0.998 6）;丹参酮ⅡA在0.074~0.222μg范围内与峰面积呈良好的线性关系（r=0.999 5）。红景天苷平均回收率为101.45%,RSD为1.78%（n=6）;丹参酮ⅡA平均回收率为101.78%,RSD为1.71%（n=6）。天香丹颗粒中红景天苷含量为1.57 mg/g,丹参酮ⅡA含量为0.58 mg/g。结论本法快捷、简便,结果准确、可靠、重复性好,可作为天香丹颗粒中红景天苷和丹参酮ⅡA的测定方法。

Objective To establish a HPLC method for determination on salidroside and tanshinone Ⅱ A in Tianxiangdan granule.Methods The HPLC system consisted of chromatographic column of Hypersil ODS2 （4.6 mm·250 mm,5 μm）;acetonitrile -0.03% phosphoric acid solution （7∶93）as mobile phase;the 220 nm detection wavelength;and 1 mL/min flow rate.Results Salidroside in the range of 5.44 -16.32 μg and the peak area had a good linear relationship （r =0.9986）;Tanshinone Ⅱ A in the range of 0.074-0.222 μg and the peak area had a good linear relationship （r =0.9995）.The average recovery rate of Salidroside was 101.45% and RSD was 1.78% （n =6）;the average recovery rate of Tanshinone Ⅱ A was 101.78% and RSD was 1.71% （n =6）.The content of Salidroside was 1.57 mg/g and Tanshinone Ⅱ A was 0.58 mg/g in Tianxiangdan granule.Conclusion This method,quick,simple,accurate,reliable,with good reproducibility,can be used as a method for the determination on salidroside and tanshinone Ⅱ A in Tianxiangdan granule .