响应面优化复合植物水解酶提取梅州金柚皮可溶性膳食纤维

Optimization of extraction of soluble dietary fiber from Meizhou gold-pomelo peel by viscozyme L

采用复合植物水解酶结合酸法提取梅州金柚皮中可溶性膳食纤维,以单因素试验为基础,以可溶性膳食纤维提取率为指标,选择酸法适合提取条件,再根据Box-Behnken实验设计原理,通过响应面分析得到酶法提取优化组合条件。得到最佳工艺为:稀硫酸p H3、物料比固定为1:20、酸解温度为50℃,酸解时间2 h,酶解温度为44℃,酶添加量为0.25 mg/g,酶解时间为2 h,此时可溶性膳食纤维提取得率为20.2438 mg/g。膳食纤维的持水力为9.397 g/g,溶胀性为11.25 m L/g,梅州金柚皮可以作为一种优良的膳食纤维来源。

Using viscozyme L with acid extraction of soluble dietary fiber in Meizhou 9old-pomelo peel, on the basis of single factor experiment, soluble dietary fiber extraction rate as index, selection of acid method for extracting conditions, according to the principle of Box-Behnken experiment design, the optimized combination is obtained by response surface analysis conditions. The best condition is that： Dilute sulfuric acid pH 3, material ratio for 1：20 fixed, acid hydrolysis temperature of 50 ~C, acid hydrolysis time 2 h, enzymolysis temperature to 43.6364 ~, the adding amount of 0.2495 mg/g, enzymolysis time was 2.0556 h, the soluble dietary fiber obtained rate is 20.2438 mg/g. Dietary fiber of a hydraulic is 9.397 g/g, swelling of 11.25 mL/g, Meizhou assembling skin can be used as a good source of dietary fiber.