摘要
免疫分析方法十分灵敏,而又具有很好的特异性。当常规的药物分析方法在样本前处理或是检测环节遇到困难时,免疫分析方法常可提供解决办法,尤其是在涉及到生物样本的前处理或生物药物浓度检测时更为重要。本文对免疫分析方法在体内药物分析中的应用进行了综述,包括生物样本前处理时的免疫萃取与免疫耗竭,以及目标药物测试过程中的各种常用的及新的免疫分析方法。
Immunoassay technology is an analytical method with high sensitivity and specificity; it provides a technique to assay materials which cannot be measured by other methods, or are difficult to detect. It plays a very important role in biological sample pre-treatment, therapeutic drug monitoring and drug determination, and is one of the important means for in vivo drug analyses. This paper reviews immunoassays commonly used in bioanalysis, including immunoextraction and immunodepletion for pretreatment of biological samples, conventional immunoassay methods and new immunoassay technologies for determination of target drugs.
基金
National Natural Science Foundation of China(Gr ant No.81102499)
Hunan Science and Technology Project(Grant No.2011SK3261)
