间充质干细胞移植肺气肿大鼠肺组织病理改变及炎性因子表达变化

Effects of MSCs transplantation on pathological changes of rats with pulmonary emphysema and expression changes of inflammatory factor

目的观察间充质干细胞(MSCs)移植后肺气肿大鼠肺组织病理改变及血清、肺组织中炎性因子水平的变化。方法雌性SD大鼠24只,随机分为对照组、模型组及移植组,各8只。模型组及移植组采用烟熏法制作肺气肿大鼠模型,对照组常规饲养。移植组予MSCs移植。MSCs移植8周后同期处死各组大鼠,取肺组织行病理检查,计算平均内衬间隔(MLI)及平均肺泡数(MAN);采用蛋白芯片技术检测大鼠血清及肺组织中的炎性因子,筛选差异性表达的炎性因子。结果移植组移植8周后于大鼠肺组织内观察到MSCs。模型组及移植组大鼠肺组织呈肺气肿样改变,且移植组较模型组轻。移植组大鼠肺组织MLI、MAN与模型组、对照组相比,P均<0.05。模型组与对照组肺组织中差异性表达的炎性因子为睫状神经营养因子(CNTF)、神经生长因子(NGF)、IL-10、激活素A、趋化因子CX3及瘦素;移植组与模型组肺组织中差异性表达的炎性因子为CNTF、IL-10及TNF-α;三组肺组织中CNTF水平相比,P均<0.05。模型组与对照组血清中差异性表达的炎性因子为CNTF、IL-1β、IL-2、IL-4及IL-6;移植组与模型组血清中差异性表达的炎性因子为IL-1β、IFN-γ及IL-10;三组血清IL-1β水平相比,P均<0.05。结论MSCs移植可减轻肺气肿大鼠肺组织病理改变,其机制可能为下调炎性因子表达。

Objective To observe the effects of transplantation of mesenehymal stem cells （ MSCs） on pathological changes of rats with pulmonary emphysema and changes in levels of inflammatory factors in the serum and lung tissues.Meth-ods Twenty-four female SD rats were randomly divided into three groups：the control group, emphysema group and trans-plantation group, 8 per group.Rat models of emphysema were established by fumigation in the emphysema group and trans-plantation group.Rats in the control group were fed routinely.MSCs were injected into recipient rats of transplantation group. Eight weeks after transplantation of MSCs, lung fissure sections were made to study the morphological alterations, mean alveo-lar numbers （ MAN） and mean linear intercept （ MLI） were calculated.The inflammatory factors in the serum and lung tis-sues were analyzed by using protein chip technique and differently expressed inflammatory factors were screened.Results In the transplantation group, 8 weeks after transplantation, MSCs can be observed in the lung tissues.Emphysematous changes of lung tissues were observed in both emphysema group and transplantation group.Emphysematous changes in transplantation group were more serious than that of the emphysema group.Significant difference was found in MLI and MAN of lung tissues in the transplantation group as compared with that of the other two groups （all P〈0.05）.The differently expressed inflamma-tory factors were ciliary neurotrophic factor （CNTF）, nerve growth factor （NGF）, IL-10, activin A, chemokine CX3 and leptin in the lung tissues between the emphysema group and control group;and the differently expressed inflammatory factors were CNTF, IL-10 and TNF-αin the lung tissues between the transplantation group and emphysema group.The levels of＆amp;nbsp;CNTF in the lung tissues were statistically different among these three groups （all P〈0.05）.The differently expressed in-flammatory factors in the serum were CNTF, IL-1β, IL-2, IL-4 and IL-6 between the emphysema group and control group;and the differently expressed inflammatory factors in the serum were IL-1β, IFN-γand IL-10 between the transplantation group and the emphysema group.The levels of IL-1βwere statistically different among these three groups （all P〈0.05）. Conclusions MSCs transplantation can effectively alleviates the pathological changes of pulmonary emphysema in rat mod-els, whose mechanism may be involved in down-regulating the expression of inflammatory factor.