摘要
Polyethylenimine-poly（L-lysine） （PEI-PLL） copolymer was synthesized via ring-opening polymerization of L-lysine N-carboxyanhydride （Lys（Z）-NCA） initiated by PEI. The complexation of PEI-PLL with siRNA was studied by particle size and zeta potential measurements. The flow cytometric analysis and confocal imaging showed its excellent intracellular trafficking ability. PEI-PLL displayed higher gene silencing efficiency and lower cytotoxicity than commercial PEI-25k in vitro. In the antitumor study, PEI-PLL was further combined with siVEGF and showed obviously tumor inhibition effect for the treatment of CT26 tumor model. Therefore, PEI-PLL is a promising siRNA carrier candidate for further antitumor treatment in vivo.
Polyethylenimine-poly（L-lysine） （PEI-PLL） copolymer was synthesized via ring-opening polymerization of L-lysine N-carboxyanhydride （Lys（Z）-NCA） initiated by PEI. The complexation of PEI-PLL with siRNA was studied by particle size and zeta potential measurements. The flow cytometric analysis and confocal imaging showed its excellent intracellular trafficking ability. PEI-PLL displayed higher gene silencing efficiency and lower cytotoxicity than commercial PEI-25k in vitro. In the antitumor study, PEI-PLL was further combined with siVEGF and showed obviously tumor inhibition effect for the treatment of CT26 tumor model. Therefore, PEI-PLL is a promising siRNA carrier candidate for further antitumor treatment in vivo.
基金
financially supported by the National Natural Science Foundation of China(Nos.51222307,51303173,51390480,21474104 and 51403205)
the Ministry of Science and Technology of China(International cooperation and communication program 2011DFR51090)
Jilin Province Science and Technology Development Program(Nos.20120306,20130521011JH)
