白藜芦醇通过促进miR-34c表达抑制人结直肠癌SW480细胞的生长

Resveratrol inhibits proliferation of colorectal cancer SW480 cells by increasing mi R-34c expression

目的探究白藜芦醇抑制人结直肠癌SW480细胞生长的可能机制。方法利用白藜芦醇(50μmol/L)处理培养的SW480细胞24 h,采用实时无标记动态细胞分析技术检测细胞增殖能力,流式细胞术检测细胞凋亡,q RT-PCR检测KITLG m RNA及mi R-34c表达,Western blot检测KITLG蛋白表达。结果与对照组比较,白藜芦醇处理后SW480细胞增殖率降低了51.5%,凋亡细胞的比例增加了343.9%;同时,白藜芦醇显著提高SW480细胞内mi R-34c的含量,与对照组相比增加了263.9%,并使其靶基因KITLG的蛋白表达水平降低52.5%。结论白藜芦醇具有明显抑制人结直肠癌SW480细胞的增殖并诱导其凋亡的作用,其可能机制与白藜芦醇增加mi R-34c表达有关。

Objective To explore the effect and molecular mechanism of Resveratrol （Res） on colorectal cancer cell line （SW480） growth. Methods The cultured SW480 cells were treated with Res for 24 h. Real time cellular analysis was performed to detect the capacity of cell proliferation. The cell apoptosis was detected by flow cytometry assay, qRT-PCR were applied to measure the levels of K/TLG mRNA and rniR-34c. Western blot was used to detect KITLG protein. Results Compared with controls, Res caused decreased cell proliferation by nearly 51.5% and the apoptosis fraction was significantly increased by 343.9%. Furthermore, Res could remarkably up-regulate the expression of miR-34c by 263.9% which knocked down its target KITLG by 52.5%. Conclusion Res could significantly inhibit the proliferation of SW480 cells and induce apoptosis. The involved molecular mechanism was probably due to the activation of miR-34c/KITLG pathway.