孕鼠葡萄糖摄入量对胎盘胰岛素样生长因子表达的影响

Pregnant rats intake different levels of glucose to influence the placental IGFs expression

目的通过孕期中给予孕鼠不同的葡萄糖摄入量,研究其胎盘中胰岛素样生长因子(IGF)表达情况,同时观察胎鼠结局。方法选取成功交配的雌性KM小鼠48只,随机分为3组:对照组(8只)、实验A组(20只)和实验B组(20只)。孕期中三组均给予正常喂养,A组每天另给予5%葡萄糖溶液1 ml灌胃,B组另给予10%葡萄糖溶液1 ml灌胃。所有孕鼠均在第19天时进行剖宫产,使用Western blot和RT-PCR方法对胎盘组织的IGF-1、IGF-2及IGF-1R进行检测,同时对胎鼠数量和畸形、死胎、存活胎情况进行观察,并对存活胎胎鼠体重和血糖水平进行检测。结果 (1)Western blot和RT-PCR检测结果显示,实验A组和实验B组孕鼠胎盘组织中IGF-1、IGF-2及IGF-1R的表达水平均高于对照组,其中实验B组中的表达水平最高(P<0.05);(2)实验A组和实验B组的胎鼠体重和血糖水平均高于对照组,其中实验B组水平最高(P<0.05);(3)三组中胎鼠发育均较正常,胎鼠数量和畸形、死胎情况未发现有明显增多(P>0.05)。结论孕鼠葡萄糖摄入量增多会诱导胎盘组织IGF-1、IGF-2及IGF-1R表达水平增加,促进胎鼠对葡萄糖的吸收利用及胎儿体重的增加。

Objective To investigate the placental IGFs expressions and fetal rats results when pregnant rats intake difference levels of glucose in pregnancy. Methods There were 48 female KM rats which had copulated successfully were selected, they were randomly divided into three groups： the control group （n=8）, the experimental group A （n=20） and the experimental group B （n=20）. During pregnancy, the three groups rats were given normal feeding, the experimental group A was given 5% glucose solution intragastric administration every day, the experimental group B was given 10% glucose solution intragastric administration every day. All the pregnant rats were given cesarean at the 19th days, Western blot and RT-PCR method were used to detect IGF-1, IGF-2 and IGF-1R of placental tissue expression levels, while the number of fetal, malformation, stillbirth and survival of fetal were observed, survival of fetal body weight and blood glucose level were detected. Results （1） Western blot and RT-PCR detecting results showed that IGF-1, IGF-2 and IGF-1R expression levels in the experimental group A and B were higher than the control group, the experimental group B was the highest （P〈0.05）; （2） Fetal rats body weight and blood glucose level in the experimental group A and B were higher than the Control group, the experimental group B was the highest （P〈0.05）; （3） Fetal rats development were normal in three groups, the number of fetal rats, malformation and stillbirth were not reach difference （P〉0.05）.Concluslon The high level glucose intake during pregnancy may result in higher placental tissue IGF^I and IGF-2 and IGF-1R expression levels, which can promote fetal rats glucose utilization and weight gain.