桦褐孔菌子实体三萜单体的分离及鉴定

Isolation and Identification of Triterpenoids in Inonotus Obliquus

利用超声破壁处理,氯仿为溶剂对桦褐孔菌子实体中三萜单体进行提取。通过薄层层析色谱(TLC)检测,用1%香草醛-浓硫酸试剂作为显色剂,反复用硅胶柱色谱对氯仿提取物进行梯度洗脱,得到两种羊毛脂烷型的三萜化合物,即化合物1(121mg)和化合物2(67mg)。采用HPLC的方法在206nm处分析其纯度,同时红外光谱(IR)分析两种化合物的特征,以及核磁共振(1 H-NMR和13 C-NMR)技术对两种羊毛脂烷型的三萜化合物进行结构鉴定。对比已知标准谱图,最终确定其结构为羊毛甾醇(Lanosterol)和栓菌酸(Trametenolic acid)。

Triterpenoid compounds in Inonotus obliquus were extracted by using CHCl3 as solvent and ultrasonic wall-breaking treatment.The thin-layer chromatography(TLC)was applied.1% vanillinsulfuric acid reagent served as the color developing agent.Gradient elution was conducted repeatedly for chloroform extracts with silica gel column chromatography to gain two types of lanostane-type triterpenoids,i.e.compound 1(121 mg)and compound 2(67 mg).Moreover,HPLC method was applied to analyze the purity of triterpenoids at 206 nm.IR was used to analyze features of the two compounds.Meanwhile,nuclear magnetic resonance(1 H-NMR and 13C-NMR)technology was used to identify the structures of two lanostane-type triterpenoids.Through comparing the known standard spectrum diagram,the structures were finally confirmed as lanosterol and trametenolic acid.