覆盆子活性成分山奈酚的含量测定

Quantitative Determination of Active Ingredient Kaempferol in Rubus Chingii Hu

目的：建立覆盆子改善肾阳虚型痴呆活性成分山奈酚的含量测定方法。方法：采用高效液相色谱法。色谱柱为Diamon－sil C18（250mm×4.6mm，5μm），流动相：乙腈－0.1％磷酸水（30∶70），流速1 mL／min，柱温30℃，检测波长266nm，进样量20μL。结果：山奈酚质量在0.0782～5.000μg范围内与峰面积呈良好的线性关系（r＝0.9999），平均加样回收率为99.50％， RSD＝2.1％。结论：试验方法简便、快速、实验结果准确可靠，该方法可作为覆盆子有效成分山奈酚含量测定的方法。

Objective：To establish method for the quantitative determination of anti-aging active ingredient kaempferol in Rubus Chin-gii Hu.Methods： Using high performance liquid chromatography.The column was Diamonsil C18 （250mm×4.6mm,5μm）,mobile phase：acetonitrile-0.1%phosphoric acid in water （30∶70）,flow rate：1mL/min, column temperature：30℃, detection wavelength：266nm, the injection volume：20μL.Results：The quality of Kaempferol ranged from 0.0782μg to 5.000μg.Peak area showed a good linear relationship （ r=0.9999） and the average sample recovery rate was 99.50%, RSD=2.1%.Conclusion：The method is simple, rapid and accurate, which can be used as an effective method for the quantitative determination of anti -aging active ingredient kaempferol in Rubus Chingii Hu.