3种炭疽菌P型ATP酶基因家族分析

The Analysis of P-type ATPases Gene Family in Three Colletotrichum Species

P型ATP酶是一类可以被ATP驱动其发生磷酸化的阳离子泵。利用TCDB(Transporter Classification Database)网站数据库中的P型ATP酶氨基酸序列对3种炭疽菌(禾谷炭疽、希金斯炭疽、胶孢炭疽)全基因组数据库进行搜索,结合结构域分析和进化树聚类分析,共找出候选P型ATP酶基因禾谷炭疽菌26个,希金斯炭疽菌37个,胶孢炭疽菌29个。对这些候选基因进行基因功能预测发现其主要参与转运Na+/K+、Ca2+、重金属离子以及磷脂的功能。利用MEME程序分析,从3种炭疽菌中均鉴定获得20个基序,其中有6个基序相同。这为深入研究炭疽菌P型ATP酶家族基因生物学功能奠定了基础。

P-type ATPases are one type of cation pumps, which can be driven by ATP to become phosphorylated. In the study, the candidate P-type ATPase genes of three Colletotrichum species were obtained by genome sequence blast using P-type ATPases amino sequence from Transporter Classification Database. In order to confirm the genes in Colletotrichum species, we analyzed the candidate P-type ATPase genes by using simple modular architecture research and phylogenetic analysis. Results showed that 26 genes were identified from C. graminicola genome database, 37 genes were identigied from C. higginsianum, and 29 genes were identified from C. gloeosporioides, and the genes mainly involved in transport of Na~/K~, Ca2~, heavy metal ions and phospholipids. And among the genes, 20 motifs and six consensus motifs were found in all three Colltotrichum specices by MEME software analyzed. The results in the paper would facilitate to further function characterized of the P-type ATPases gene family in Colletotrichum species.