双瓣茉莉5个花期挥发性成分GC-MS分析

Volatile Components Analysis of Five Flower Phases of Jasminum sambac cv. Bifoliatum by GC-MS

采用气质联用仪(GC-MS)对福州双瓣茉莉花小花蕾期、大花蕾期、花开12 h开花期、花开24 h开花期和花败期5个时期挥发性成分进行鉴定,并用峰面积归一化法分析各组分含量。结果表明,开花期是挥发性物质集中释放期,花败期含量急剧下降,小花蕾和大花蕾时期仅少量释放,这符合茉莉花为气质花的特性。萜类和酯类挥发性物质是双瓣茉莉花香主要成分,萜类挥发性物质在盛开时大量释放,酯类挥发性物质通常在花开12 h开花期释放量最高。5个不同时期共鉴定出43种挥发性成分,挥发性物质种类和绝大部分挥发性物质含量均是在开花期最高,其中含量较高的乙酸芳樟酯、α-金合欢烯、乙酸苯甲酯、苯甲醇、γ-依兰油烯、乙酸甲酯、反式-法呢醇和β-罗勒烯等是双瓣茉莉挥发性物质的主要成分。结果将为茉莉花精油的提取及茉莉花制茶的采摘时期提供依据,为开展双瓣茉莉花挥发性物质代谢途径的分子调控机理研究奠定基础。

The volatile Compounds were extracted from early bud, mature bud, flowered 12 hours after, flowered 24 hours later and trockne blumen of Jasminum sambac cv. Bifoliatum, and were identified by Gas ChromatographyMass Spectrometer（GC-MS）. The relative percentage contents were carried out by peak area normalization method.Research results： Anthesis stage was a main time of the compounds volatilization, which decreased sharply in trockne blumen period, only a few of which existed in early and mature bud. The findings were consistent with jasmine characteristics of little frangrance in non-blossoming. The main kinds of volatile ingredient of dual-petal jasmine were terpenes and esters, terpenoids volatilized largely in flowering, and esters reached maximum volatilization in that flowered 12 hours after. 43 volatile compounds were identified from 5 different times, the kinds and content of which reached maximum in blooming, and the main volatile compounds included linalyl acetate, α-farnesene, methyl phenylacetate, benzyl alcohol, γ-muurolene, acetic acid methyl ester, trans-Farnesol and β-Ocimene. Research significance： The test data from the study would provide a basis for the extraction of jasmine essential oil, and provide the reference for the harvest period of jasmine tea, and also support the research on the molecular regulation mechanism of volatile substances metabolic pathways of Jasminum sambac cv. Bifoliatum.