不同采收期广佛手指纹图谱研究

HPLC Fingerprint of FRUCTUS CITRI SARCODACTYLIS in Different Picking Times

[目的]建立不同采收期的广佛手指纹图谱,探索广佛手的最佳采收期。[方法]采用RP-HPLC法,Hypersil C18(250 mm×4.6mm,5μm)色谱柱,流动相为甲醇-0.05%磷酸水溶液,梯度洗脱;流速1.0 ml/min,柱温30℃,检测波长283 nm。[结果]不同采收期广佛手成分的种类及含量存在一定的差异,9月份样品整体相似度高于7月份样品,广佛手以9月份采收为宜。[结论]该方法简便可行、重复性较好,可为质量控制以及实现规范化种植提供可靠的科学依据。

[ Objective] To establish HPLC fingerprints of FRUCTUS CITRI SARCODACTYLIS in different picking times, and explore the best harvest time of FRUCTUS CITRI SARCODACTYLIS. [ Method] HPLC chromatography condition： Hypersil C18 column （250 mm×4.6mm,5μm） ; the mobile phase was acetonitrile with 0.05% phosphoric acid and the gradient elution mode was applied in chromatographic separation; The flow rate was 1.0 ml/min; The temperature of column was 30 ℃ ; The detective wavelength was set at 283 nm. [ Result] There was some difference of the type and content of composition of FRUCTUS CITRIS SARCODACTYLIS in different picking times. The overall similarity in samples collected in September was higher than that in July, and September was the appropriate for harvest. [ Conclusion ] The method is simple and feasible, has good repeatability, and can provide reliable scientific basis for quality control and standardized cultivation.