HBV感染者血清抗TRP-1抗体与HSP70检测及相关性分析

Detection of Serum Autoantibodies to Tyrosinase-related Protein-1 and HSP70 in Patients with HBV

目的检测HBV感染者血清抗TRP-1抗体及HSP70,并对二者的相关性进行分析。方法选取HBV感染组血清标本88例,其中大三阳组28例,小三阳组60例;抗体阳性组标本64例,其中既往感染组32例,疫苗接种组32例;全阴性对照组64例。用ELISA法检测所有标本抗TRP-1抗体及HSP70表达水平,对各组检测结果进行统计并分析两者的相关性。结果大三阳组、小三阳组、既往感染组、疫苗接种组、全阴性对照组血清标本中抗TRP-1抗体的OD值依次为2.071±0.574、1.855±0.873、0.166±0.068、0.220±0.048和0.260±0.034,抗TRP-1抗体阳性率分别为92.86%(26/28)、83.33%(50/60)、0%(0/32)、0%(0/32)和0%(0/64)。HSP70检测的OD值分别为2.135±0.456、1.816±0.874、0.165±0.073、0.166±0.042和0.206±0.033,高水平的HSP70占有率分别为100%(28/28)、83.33%(50/60)、0%(0/32)、0%(0/32)和0%(0/64)。经比较HBV感染组抗TRP-1抗体与HSP70表达水平显著高于抗体组及全阴性对照组(P<0.01)。高水平的抗TRP-1抗体与HSP70表达水平呈正相关(r=0.926,P<0.01)。抗体组与全阴性对照组之间比较,差异无统计学意义(P>0.05)。结论大部分HBV感染者体内同时存在高水平抗TRP-1抗体及HSP70,两者呈正相关,可能是该类患者黑素细胞遭受破坏的早期标志,也是继发白癜风的重要原因。

Objective To detect serum autoantibodies against tyrosinase related protein-1 （a-TRP-1 ）and heat shock protein 70 （HSP70）in patients with HBV and analysis their relationship. Methods The serum a-TRP-1 and HSP70 were detected by ELISA method in 216 patients with HBV including 28 subjects with HbsAg, HbeAg and HbcAb positive（ major three positive group）, 60 subjects with HbsAg, HbeAb and HbcAb positive （minor three positive group）, 32 subjects with HbsAb, HbeAb and HbeAb positive（past infected group）, 32 subjects with HbsAb positive（ vaccinated group）and 64 subjects with all negative （negative group）. Results The serum levels of a- TRP- 1 in each group were 2. 071 ± 0. 574, 1. 855 ± 0. 873, 0. 166± 0. 068, 0. 220±0. 048 and 0. 260±0. 034 respectively. The positive rates of high level a-TRP-1 were 92.86% （26/28）,83.33% （50/60）, 0% （0/32）, 0% （0/32）and 0% （0/64）. The serum levels of HSP70 in each group were 2. 135 ±0. 456,1. 816 ± 0. 874,0. 165 ± 0. 073,0. 166± 0. 042 and 0. 206± 0. 033 respectively. The positive rates of high level HSPT0 were 100% （28/28）, 83.33% （50/60）, 0% （0/32）, 0% （0/32）and 0% （0/64）. The levels of a-TRP-1 and HSP70 in subjects infected by HBV were significantly higher than that of other groups （P 〈0.01 ） , while there were no significant difference in the past infected group, the vaccinated group and the negative group （ P 〉 0.05 ）. High levels of HSP70 and a- TRP- 1 were positive correlated （ r = 0. 926, P 〈 0.01）. Conclusion High levels of a-TRP-1 and HSPT0 can be detected in sera of most patients infected by HBV and they are positive correlated, which may be early signs of melanocyte destruction and the reasons for the cause of vitiligo.