湖北省人乳头瘤病毒基因分型检测分析

Analysis of Human Papillomavirus Infection and Typing in Hubei Province

目的调查湖北省女性人群人乳头状瘤病毒(human papillomavirus,HPV)感染状况,为宫颈癌防治提供参考依据。方法收集2013年1月至2014年11月在湖北省中山医院就诊的3956例宫颈脱落细胞标本,采用PCR体外扩增和DNA反向点杂交相结合的DNA芯片技术,对宫颈脱落细胞标本进行HPV基因分型检测。结果 3956例患者中共检出HPV阳性818例,阳性率为20.68%,其中高危型HPV感染阳性率为15.77%(624/3956)。在被检测的23个HPV-DNA亚型中,最常见的依次为16型(3.72%,147/3956),52型(3.67%,145/3956),43型(3.64%,144/3956),58型(2.78%,110/3956),18型(1.49%,59/3956),33型(1.06%,42/3956),未检出MM4型。HPV阳性者中多重感染率为17.36%(142/818),以二重感染最常见(85.21%,121/142)。结论湖北省地区高危型HPV感染以HPV16、52、58、18和33比较常见,低危型HPV感染以HPV43比较常见。

Objective To investigate HPV infection status of women in Hubei province in order to provide the basis for the prevention and treatment of the cervical cancer. Methods The infection status of HPV in 3956 patients of gynecology department was examined with gene chip technique in Hubei Zhongshan hospital from 2013 January to 2014 November. Results Among the 3956 patients, 818 patients were infected with HPV （20.68%）, and 624 patients were infected with HR-HPV（high risk HPV, 15.77% ）. Out of the 23 subtypes of HPV, HPV-16 （3.72%, 147/3956） was the most common of all types, followed by subtypes HPV-52 （3.67% ,145/3956）, HPV-43 （3.64%, 144/3956）, HPV-58（2.78%, 110/3956）, HPV-18（1.49%, 59/3956）, HPV-33（1.06%, 42/3956）, and no HPV-MM4. In the HPV positive patients, 142（17.36%, 142/818） patients were compound infected, of whom 121 （ 85.21% , 121/142） patients were super- infected. Conclusion The more common high-risk HPV subtype are HPV16, HPV52, HPV58, HPV18 and HPV33 and low-risk HPV subtype is HPV43 in Hubei province.