骨髓间充质干细胞-脱细胞真皮基质对大鼠表皮缺损的修复

Acellular dermal matrix with bone marrow mesenchymal stem cells for the repair of skin defect in SD rat

目的研究骨髓间充质干细胞(bone marrow mesenchymal stem cell,BMSC)-脱细胞真皮基质(acellular dermal matrix,ADM)对SD大鼠皮肤表皮缺损的修复。方法培养SD大鼠骨髓细胞,增殖提纯传代至4代时,成骨成脂,流式细胞仪检测鉴定BMSC,然后将其接种到ADM,2d后移植到皮肤表皮缺损的SD大鼠体内,连续5周观察其修复效果。结果 BMSC-ADM修复SD大鼠皮肤表皮缺损效果良好,5周后,肉眼可见材料与组织结合紧密,有弹性,颜色接近皮肤。结论 BMSC-ADM可以作为修复SD大鼠皮肤表皮缺损的组织工程材料。

Objective To investigate acellular dermal matrix（ ADM ） combined with bone marrow mesenchymal stem cells（ BMSC）for the repalr of skin defects in SD rat. Methods We identified BMSCs by flow cytometry after the bone marrow cells of SD rats were cultured,proliferated,purified,passaged,and then we inoculated BMSCs into ADM. After two days,the ADM combined with BMSC was transplanted into the skin defect of SD rats to observe the repalring effect for five weeks. Results The repalring effect of ADM combined with BMSC in the skin defect of SD rats was good. The visible material was linked closely and flexibly with the organization of SD rats,and the color was closed to the normal skin after five weeks. Conclusions ADM combined with BMSC could be used as a tissue engineering material for repalring the skin defect of SD rats.