儿童感染性腹泻病原菌分布及耐药分析

Distribution and Drug Resistance of Pathogens in Children With Infectious Diarrhea

目的观察儿童感染性腹泻病原菌的分布情况,并对其耐药性进行分析。方法选取我院收治的培养出病原菌的1 572例感染性腹泻患儿作为临床研究对象,取其大便标本进行常规的病原菌分离培养,采用ATP细菌鉴定仪对细菌进行鉴定,然后通过诊断血清凝集进行确认和分型,最后使用K-B纸片扩散法进行药敏试验分析。结果 1 572例感染性腹泻患儿的大便标本中,共检出84株病原菌,阳性率为5.3%。20株大肠埃希菌对于多种抗菌药物均普遍具有耐药性,其中对于氨苄西林和哌拉西林的耐药率均非常高,共检出6株ESBL菌株,共占30.00%。沙门菌和志贺氏菌对于第3代头孢菌素和头孢哌酮/舒巴坦为基本敏感。所有菌株对于哌拉西林/他唑巴坦、亚胺培南类药物均比较敏感。结论儿童感染性腹泻应根据病原菌的分布及耐药性情况来选择合理的抗菌药物。

Objective To observe the distribution of pathogenic bacteria in children infectious diarrhea and investigate the drag resistance analysis. Methods Chose 1572 children with infectious diarrhea who were cultured pathogens as research objects. Took the stool specimens for routine pathogen isolation and culture, the bacteria identiifcation was used by ATP automatic detection machine, and then through the diagnosis of serum agglutination to get recognition and classifications. Finally, K-B method was used to analysis of drug sensitivity test. Results In 1572 cases of stool specimens in children with infectious diarrhea, 84 strains of pathogenic bacteria were detected, the positive rate was 5.3%. 20 strains of Escherichia coli are generally resistant to many kinds of antimicrobial agents, especially resistance to ampicillin and piperacillin were very high, a total of 6 of ESBL strain detection accounted for 30.00%.The third generation cephalosporins and cefoperazone/sulbactam were basically sensitive to both Salmonella and Shigella. All bacterial strains were sensitive to Piperacillin/azobactam and Imipenem. Conclusion Choosing rational antibiotics should be according to the distribution and drug resistance of pathogenic bacteria to treat children infectious diarrhea .