正丁胺对人肝癌细胞Bel-7402增殖的影响

Effects of N-Butylamine on the Proliferation of Human Bel-7402 Hepatocarcinoma Cell Line

目的研初步检测正丁胺作用下人肝癌Bel-7402细胞的增殖情况。方法体外培养人肝细胞癌Bel-7402细胞,予不同浓度正丁胺进行干预作为正丁胺干预组,无药物组作为空白对照组。绘制细胞生长曲线,并利用MTT体外检测法探讨该药物对Bel-7402细胞的半致死量,进行了初步的体外活性评价。结果较低浓度的正丁胺能够促进肿瘤细胞增殖,正丁胺浓度高于2 mmol/L时,细胞增殖速度减缓。结论当正丁胺达到一定浓度时才具有一定的体外肿瘤生长抑制作用。

Objective To investigate the effects of n-Butylamine in human hepatocellular carcinoma Bel-7402 cells. Methods Human hepatocellular cancer Bel-7402 cells were cultured in vitro, they were treated with diferent concentrations of n-Butylamine as trial group and without n-Butylamine as control group. Cell growth curve was done, and the cell viability was measured by MTT assay. Results Promoting Bel-7402 proliferation when the n-butylamine at lower concentrations, cell proliferation slowed down when the concentrations of n-butylamine was higher than 2mmol/L. Conclusion N-Butylamine reaches a certain concentration can inhibit the growth of tumor in vitro.