疑似强直性脊柱炎患者HLA-B27检测与分析

Detection and analysis of HLA-B27 antigen in patients with suspected ankylosing spondylitis

目的通过对872例强直性脊柱炎(AS)疑似患者HLA-B27抗原的检测,分析HLA-B27抗原与AS的相关性及其临床意义。方法选取872例AS疑似患者外周血,采用流式细胞仪检测T淋巴细胞膜上的HLA-B27/B7抗原,并对患者的表达情况进行分析。结果 872例AS疑似患者男女比例为1.8∶1,HLA-B27抗原总阳性率为27.29%,,男女患者阳性率分别为32.50%和17.95%(P<0.05),男女患者B27+/B7-单抗表达率(%)分别为39.16±42.79和20.96±33.86(P<0.05),平均荧光强度分别为5.35±5.44和3.35±3.87(P<0.05),差别显著。结论用流式细胞技术检测T淋巴细胞HLA-B27的有助于AS的诊断和鉴别诊断。

Objective To investigate the relationship between suspected ankylosing spondylitis and HLA‐B27 antigen by detec‐ting the positive frequency of HLA‐B27antigen in 872 suspected AS patients ,and evaluate its clinical significance .Methods The positive frequency of HLA‐B27 on the T lymphocyte membrane were detected by flow eytometer in 872 suspected AS patients .Re‐sults Among the 872 suspected AS patients the ratio between male and female was 1 .8∶1 ,the positive rate of antigen HLA‐B27 was 27 .29% ,and the male and female patients′positive rates of HLA‐B27 antigen were 32 .50% and 17 .95% ,respectively （P〈0 .05） .The male and female patients′ expression percentage of B27^＋ /B7^- monoclonal antibody were 39 .16 ± 42 .79 and 20 .96 ± 33 .86 ,respectively（P〈0 .05） .The male and female patients′mean fluorescence intension of B27＋ /B7- monoclonal antibody were 5 .35 ± 5 .44 and 3 .35 ± 3 .87 ,respectively（P〈0 .05） .Conclusion The patients with AS are strongly associated with HLA‐B27 an‐tigen .Detection of HLA‐B27 antigen expression intensity in suspected AS patients with FCM is helpful to diagnosis and differential diagnosis of AS .