肝硬化并肝细胞癌的端粒酶活性和氧化应激研究

Study of telomerase activity and oxidative stress in hepatocellular carcinoma with cirrhosis

为阐明端粒酶活性和氧化应激在肝细胞癌 (HCC)合并肝硬化及肝硬化患者组织中的表达、相互关系及意义 ,采用 TRAP- EL L ISA方法测定了 2 1例 HCC合并肝硬化 (下称 HCC组 )和 2 3例肝硬化 (下称肝硬化组 )患者组织中的端粒酶活性 ,并用生化法测定其组织中的丙二醛 (MDA)、谷胱甘肽 - S转移酶 (GST)和总抗氧化能力 (T- AOC)。结果显示 ,HCC组端粒酶阳性 18例 ,阴性 3例 ;肝硬化组阳性 3例 ,阴性 2 0例 ,两者差别有显著意义 (P<0 .0 0 1) ;MDA、GST和 T- AOC在两组中的表达均有显著差异 (P均 <0 .0 0 1) ;端粒酶活性和 MDA含量呈正相关 (P<0 .0 5 )。认为从肝硬化到肝癌的过程与机体抗氧化系统功能失调密切相关 ;端粒酶激活是 HCC癌变的早期事件 ;氧化应激对端粒酶激活可能起重要作用。

To study the relationship between telomerase activity and oxidative stress in hepatocellular carcinoma with cirrhosis(HCC),TRAP-ELISA assay were used to determine telomerase activity in 21 cases of HCCc as well as in 23 cases of cirrhosis,the expression of malondialdehyde,glutathione S-transferase and total anti-oxidative capacity were also examined in the same samples.18 of 21 cases of HCC were found to have increased telomerase activity ,whereas only 3 out of the 23 non-cancerous cirrhosic samples were found to have weak telomerase activity, the difference was significant(P<0.001). There were significant differences between HCCc and cirrhosis in the expression of malondialdehyde, glutathione S-transferase and total anti-oxidative capacity respectively.Telomerase activity was correlated positively with the content of MDA(P<0.05).These suggest that the dysfunction of the anti-oxidative system is closely correlated with the progression from cirrhosis to hepatocellular carcinoma,telomerase activation is the early event of carcinogenesis,oxidative stress may contribute partly to telomerase activation.