外周血可溶性E-选择素与慢性乙型肝炎的关系

Relation between serum soluble Eselectin and chronic hepatitis

为研究慢性乙型肝炎 (慢乙肝 )患者血清可溶性 E-选择素 (s E- selectin)与肝功能、肝纤维化指标及血清病毒载量的关系 ,采用双抗体夹心 EL ISA法检测了 5 4例慢乙肝患者的血清 s E- selectin水平 ,用荧光定量聚合酶链反应 (PCR)检测其血清 HBVDNA定量 ,用放射免疫法测定其透明质酸 (HA)、 型前胶原 (PC ) ,同时检测肝功能等。结果显示 ,慢乙肝患者外周血 s E- selectin水平明显高于正常对照组 (P<0 .0 0 1) ,s E- selectin水平与丙氨酸转氨酶 (AL T)、天门冬氨酸转氨酶 (AST)、总胆红素 (TBIL)呈正相关 (P均 <0 .0 1) ,与白蛋白 (AL B)呈负相关 (P<0 .0 1) ;与血清 HA、PC 水平均呈正相关 (P均 <0 .0 1) ;与血清 HBV DNA定量无相关性 (P>0 .0 5 )。认为 s E- selectin水平可反映慢乙肝患者的炎症活动度和肝纤维化程度 ,不能反映其病毒血症水平。

In order to investigate the changes of serum soluble E-selectin (sE-selectin) in patients with chronic hepatitis B (CHB) , sE-selectin was measured by an enzyme-linked immunosorbent assay in the sera of 54 patients with CH-B and 14 healthy controls. The liver founction, serum levels of hyaluronic acid (HA ) , typeⅢ procollagen (PCⅢ) and the quantitation of HBV DNA were also detected in the same time . As a result, the serum level of sE-selectin in patients with CHB was higher significantly than that of healthy controls ( P< 0.01) . The sE-selectin concentration had a correlation with ALT, AST and TBIL levels ( P<0.01, respectively ). The sE-selectin concentration correlated with HA level as well as PCⅢ( P<0.01?<0.01) . But HBV DNA quantitation had no relation with the serum level of sE-selectin ( P>0.05) . The data indicates that sE-selectin concentration is a valuable detection index to reflect the inflammation and fibrosis degree but can not reflect HBVDNA level in patients with CHB.